Yang Jeong-Yeh, Della-Fera Mary Anne, Rayalam Srujana, Ambati Suresh, Baile Clifton A
Department of Animal and Dairy Science, University of Georgia, Athens, GA 30602-2771, USA.
Biofactors. 2007;30(3):159-69. doi: 10.1002/biof.5520300303.
Genistein (G), an isoflavone, and guggulsterone (GS), the active substance in guggulipid, have been reported to possess therapeutic effects for obesity. In the present study, we investigated the effects of combinations of G plus GS on apoptosis and adipogenesis in 3T3-L1 cells. In mature adipocytes, G and GS individually caused apoptosis, but combination of G plus GS significantly increased apoptosis, more than either compound alone. Furthermore, G plus GS caused a greater increase in procaspase-3 cleavage, Bax expression, cytochrome c release, and proteolytic cleavage of PARP than either compound alone. In maturing preadipocytes G and GS each suppressed lipid accumulation, but the combination potentiated the inhibition of lipid accumulation. These results suggest that combination of genistein and guggulsterone may exert anti-obesity effects by inhibiting adipogenesis and inducing apoptosis in adipocytes.
染料木黄酮（G）是一种异黄酮，而古古勒甾酮（GS）是古古勒脂中的活性物质，据报道它们对肥胖具有治疗作用。在本研究中，我们研究了G加GS组合对3T3-L1细胞凋亡和脂肪生成的影响。在成熟脂肪细胞中，G和GS单独均可引起细胞凋亡，但G加GS组合显著增加了细胞凋亡，比单独使用任何一种化合物的效果都更明显。此外，G加GS比单独使用任何一种化合物更能显著增加前半胱天冬酶-3的切割、Bax表达、细胞色素c释放以及PARP的蛋白水解切割。在正在成熟的前脂肪细胞中，G和GS各自均抑制脂质积累，但二者组合可增强对脂质积累的抑制作用。这些结果表明，染料木黄酮和古古勒甾酮的组合可能通过抑制脂肪生成和诱导脂肪细胞凋亡来发挥抗肥胖作用。
