Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso.
Malar J. 2009 Oct 26;8:239. doi: 10.1186/1475-2875-8-239.
Previous studies of Anopheles funestus chromosomal inversion polymorphisms in Burkina Faso showed large departures from Hardy-Weinberg equilibrium and linkage disequilibrium among inversions located on different chromosomes, implying the existence of two taxonomic units ("chromosomal forms") with limited genetic flow. One chromosomal form, named Folonzo, is highly polymorphic for alternative rearrangements of 3Ra, 3Rb, 2Ra, and 3La; the other, Kiribina, is predominantly characterized by the standard arrangement of these inversions. To investigate the temporal distribution of these chromosomal forms, further collections were carried out in two villages near Ouagadougou where they are found in sympatry.
Chromosomal karyotypes were determined from indoor-resting, half-gravid females sampled within and across six breeding seasons, from December 1998 to April 2007.
As expected, the pattern of chromosomal polymorphism in An. funestus was consistent with assortatively mating Folonzo and Kiribina forms. When samples were assigned to each chromosomal form, their relative abundance varied within successive breeding seasons in a repeating pattern of temporal variability. Relative abundance of the Folonzo form was correlated with climatic variables related to temperature and rainfall.
The relative abundance of Folonzo and Kiribina forms of An. funestus likely reflects different larval ecologies that are linked to varying climatic conditions. Further analysis of the bionomics of these vectors is recommended in light of its relevance to vector control.
先前在布基纳法索对致倦库蚊染色体倒位多态性的研究表明，位于不同染色体上的倒位之间存在较大的偏离 Hardy-Weinberg 平衡和连锁不平衡，这意味着存在两个分类单元（“染色体形式”），它们之间的遗传交流有限。一种染色体形式称为 Folonzo，其 3Ra、3Rb、2Ra 和 3La 的替代重排高度多态；另一种染色体形式称为 Kiribina，主要特征是这些倒位的标准排列。为了研究这些染色体形式的时间分布，在布基纳法索瓦加杜古附近的两个村庄进行了进一步的采集，这些村庄中它们是同域分布的。
从 1998 年 12 月至 2007 年 4 月的六个繁殖季节内和跨季节采集的室内休息、半饱血雌性个体中确定了染色体核型。
正如预期的那样，致倦库蚊的染色体多态模式与 Folonzo 和 Kiribina 形式的交配选择性一致。当将样本分配到每个染色体形式时，它们的相对丰度在连续的繁殖季节内以重复的时间变化模式发生变化。Folonzo 形式的相对丰度与与温度和降雨有关的气候变量相关。
致倦库蚊 Folonzo 和 Kiribina 形式的相对丰度可能反映了不同的幼虫生态，这些生态与不同的气候条件有关。鉴于其对病媒控制的相关性，建议进一步分析这些病媒的生物学特性。
