Szewczyk M, Wielkoszyński T, Zakliczyński M, Zembala M
Silesian Center for Heart Diseases, ul. Szpitalna 2, 41-800 Zabrze, Poland.
Transplant Proc. 2009 Oct;41(8):3242-3. doi: 10.1016/j.transproceed.2009.08.018.
Kidney injury represents a major clinical problem in orthotopic heart transplant (OHT) patients which seriously increases the mortality rate. The aim of this work was to evaluate the utility of a new kidney damage marker-neutrophil gelatinase-associated lipocalin (NGAL)-and its correlations with cystatin C, creatinine, and glomerular filtration rate (GFR) among patients after heart or lung transplantation.
We examined serum samples from 71 patients after heart transplantation, 7 patients after lung transplantation, and 20 healthy controls to measure serum NGAL using an enzyme-linked immunosorbent assay (ELISA) method; cystatin C using latex immunonephelometry; and creatinine using the alkaline-picrate method.
Serum NGAL levels were significantly elevated among transplant patients, but did not significantly correlate with cystatin C in the transplant group (R = .13; P = .11) or in the control group (R = .26; P = .26), or GFR in the transplant group (R = -.09; P = .25) or in the control cohort (R = .22; P = .36). In the transplant group, serum NGAL positively correlated with serum creatinine (R = .27; P = .001).
Plasma NGAL was not a specific biomarker for monitoring chronic renal disorders. We did not exclude other pathologies that might contribute to increased serum NGAL levels.
肾损伤是原位心脏移植（OHT）患者面临的一个主要临床问题，会严重提高死亡率。本研究的目的是评估一种新的肾损伤标志物——中性粒细胞明胶酶相关脂质运载蛋白（NGAL）的效用，以及它与心脏或肺移植患者的胱抑素C、肌酐和肾小球滤过率（GFR）之间的相关性。
我们检测了71例心脏移植患者、7例肺移植患者以及20名健康对照者的血清样本，采用酶联免疫吸附测定（ELISA）法检测血清NGAL；采用乳胶免疫比浊法检测胱抑素C；采用碱性苦味酸盐法检测肌酐。
移植患者的血清NGAL水平显著升高，但在移植组（R = 0.13；P = 0.11）或对照组（R = 0.26；P = 0.26）中，血清NGAL与胱抑素C无显著相关性，在移植组（R = -0.09；P = 0.25）或对照队列中（R = 0.22；P = 0.36），血清NGAL与GFR也无显著相关性。在移植组中，血清NGAL与血清肌酐呈正相关（R = 0.27；P = 0.001）。
血浆NGAL不是监测慢性肾脏疾病的特异性生物标志物。我们并未排除其他可能导致血清NGAL水平升高的病理情况。
