移植病房患者中耐万古霉素粪肠球菌的分子流行病学
Molecular epidemiology of vancomycin-resistant Enterococcus faecalis among patients of transplantology wards.
作者信息
Grzybowska W, Mlynarczyk A, Mrowka A, Tyski S, Buczkowska T, Pazik J, Durlik M, Kwiatkowski A, Adadyński L, Chmura A, Paczek L, Mlynarczyk G
机构信息
Department of Antibiotics and Microbiology, National Medicines Institute, Warsaw, Poland.
出版信息
Transplant Proc. 2009 Oct;41(8):3256-7. doi: 10.1016/j.transproceed.2009.08.029.
OBJECTIVE
Vancomycin-resistant enterococci (VRE) require epidemiological monitoring especially in transplantation wards. The aim of our work was to perform a molecular analysis of vancomycin-resistant Enterococcus faecalis (VREfl) strains among solid organ recipients during various years.
MATERIALS AND METHODS
Strains were examined for the presence of different genes determining vancomycin resistance: vanA, vanB, vanD, or vanG by polymerase chain reaction (PCR). Restriction fragment length polymorphism (RFLP)-pulsed field gel electrophoresis (PFGE) was performed on bacterial DNA digested with SmaI enzyme.
RESULTS
From 2003 to 2006, we isolated 12 strains of VREfl from 8 patients (2 liver and 6 kidney transplantations). All strains harbored the vanA gene. Among the strains, 5 displayed patterns similar to each other, despite being isolated from different patients, and were susceptible to ampicillin with high resistance to aminoglycosides.
CONCLUSIONS
These results suggested that a single strain of VREfl was present for 3 years in closely related hospital wards, but it disappeared in the following years.
目的
耐万古霉素肠球菌（VRE）需要进行流行病学监测，尤其是在移植病房。我们研究的目的是对不同年份实体器官接受者中的耐万古霉素粪肠球菌（VREfl）菌株进行分子分析。
材料与方法
通过聚合酶链反应（PCR）检测菌株中决定万古霉素耐药性的不同基因：vanA、vanB、vanD或vanG的存在情况。对用SmaI酶消化的细菌DNA进行限制性片段长度多态性（RFLP）-脉冲场凝胶电泳（PFGE）分析。
结果
2003年至2006年期间，我们从8例患者（2例肝移植和6例肾移植）中分离出12株VREfl菌株。所有菌株均携带vanA基因。在这些菌株中，5株尽管分离自不同患者，但显示出彼此相似的图谱，对氨苄西林敏感，对氨基糖苷类高度耐药。
结论
这些结果表明，一株VREfl在密切相关的医院病房中存在了3年，但在随后的几年中消失了。
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