Mlynarczyk A, Grzybowska W, Mrowka A, Tyski S, Buczkowska T, Pazik J, Durlik M, Kwiatkowski A, Adadynski L, Chmura A, Paczek L, Mlynarczyk G
Department of Medical Microbiology, Transplantation Institute, The Medical University of Warsaw, Warsaw, Poland.
Transplant Proc. 2009 Oct;41(8):3261-3. doi: 10.1016/j.transproceed.2009.08.012.
The aim of the investigations was to compare the vancomycin-resistant Enterococcus faecium (VREfm) strains obtained from our patients.
Strains were compared using restriction fragment length polymorphism-pulsed field gel electrophoresis (RFLP-PFGE) of bacterial DNA.
VREfm infected 26 liver recipients, 22 kidney recipients, and 9 other surgery or nephrology patients. Only five strains possessed the vanB determinant. The PFGE analysis revealed two large and several small groups of related strains.
The PFGE analysis enabled the investigation of VRE epidemiology among patients after transplantation. Strains with similar patterns most probably originated from one source and clearly suggested an outbreak.
调查的目的是比较从我们的患者中分离出的耐万古霉素屎肠球菌（VREfm）菌株。
使用细菌DNA的限制性片段长度多态性-脉冲场凝胶电泳（RFLP-PFGE）对菌株进行比较。
VREfm感染了26名肝移植受者、22名肾移植受者以及9名其他外科手术或肾病患者。只有五株菌株携带vanB决定簇。PFGE分析显示有两个大的相关菌株组和几个小的相关菌株组。
PFGE分析有助于调查移植后患者中的VRE流行病学。具有相似图谱的菌株很可能来自同一来源，明显提示有暴发情况。
