Azarpira Negar, Malekhosseini Seyed Ali, Aghdaie Mahdokht Hosein, Daraie Masumeh
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Exp Clin Transplant. 2010 Sep;8(3):210-3.
Cytotoxic T-lymphocyte antigen 4 (CTLA4) has a critical role in the down-regulation of the immune response. We retrospectively examined the association between acute rejection and the single nucleotide polymorphism A/G in the CTLA-4 CT60 gene in liver transplant recipients.
Fifty-one liver transplant recipients with at least 3 months' follow-up were selected and genotyped for CTLA-4 CT60 polymorphism (HpyCH4 IV). The association of each genotype with allograft acute rejection was evaluated.
The mean age of patients was 27.9 +/- 15.17 years (minimum, 1 year, maximum, 55 years), with 39% male and 61% female. Overall, 17 recipients (33.3%) experienced acute rejection within the first 3 months after a liver transplant. In our study, 50% of the patients (n=26) have G/A , 31% (n=16) have A/A, and 17% have G/G genotypes (n=9). Distribution of alleles was not different according to underlying liver disease. There also was no difference in sex, age, and distributions of CTLA-4 CT60 alleles with acute rejection episodes.
CT60 A/G dimorphism within the 3'-UTR of CTLA4 gene does not influence acute rejection development in liver transplant. However, organ rejection is determined by a combination of several genetic traits rather than a single gene. Therefore, more studies with larger patient numbers are necessary to investigate the effect of combinations of genetic phenotypes involved in this process.
细胞毒性T淋巴细胞抗原4（CTLA4）在免疫反应的下调中起关键作用。我们回顾性研究了肝移植受者急性排斥反应与CTLA-4 CT60基因单核苷酸多态性A/G之间的关联。
选择51例至少随访3个月的肝移植受者，对CTLA-4 CT60多态性（HpyCH4 IV）进行基因分型。评估每种基因型与同种异体移植急性排斥反应的关联。
患者的平均年龄为27.9±15.17岁（最小1岁，最大55岁），男性占39%，女性占61%。总体而言，17例受者（33.3%）在肝移植后的前3个月内发生了急性排斥反应。在我们的研究中，50%的患者（n = 26）为G/A基因型，31%（n = 16）为A/A基因型，17%为G/G基因型（n = 9）。等位基因的分布根据潜在的肝脏疾病没有差异。CTLA-4 CT60等位基因在性别、年龄和急性排斥反应发作方面的分布也没有差异。
CTLA4基因3'-UTR内的CT60 A/G二态性不影响肝移植中急性排斥反应的发生。然而，器官排斥反应是由多种遗传特征而非单一基因共同决定的。因此，需要更多纳入更大患者数量的研究来调查参与此过程的遗传表型组合的影响。
