Simmons D T, Takemoto K K, Martin M A
J Virol. 1977 Oct;24(1):319-25. doi: 10.1128/JVI.24.1.319-325.1977.
The monomer form of BK virus (BKV) tumor antigen (T Ag) was immunoprecipitated from extracts of BKV-transformed cells and had a molecular weight of approximately 113,000. This compared with 97,000 for the molecular weight of either BKV or simian virus 40 (SV40) T Ag from lytically infected cells. The SV40 and BKV T Ag's from productively infected cells were compared by examining their methionine-labeled tryptic peptides. Out of a total of 20 SV40-and 21 BKV-specific peptides, there were seven pairs of similar peptides on the basis of ion-exchange chromatography, These coeluting peptides contained approximately 25 to 30% of the total methionine radioactivity. Similar results were obtained when the tryptic peptides of SV40 T Ag from lytically infected cells were compared with those of BKV T Ag from virally transformed cells.
从BK病毒（BKV）转化细胞的提取物中免疫沉淀出BKV肿瘤抗原（T Ag）的单体形式，其分子量约为113,000。相比之下，来自裂解感染细胞的BKV或猴病毒40（SV40）T Ag的分子量为97,000。通过检查其甲硫氨酸标记的胰蛋白酶肽来比较来自生产性感染细胞的SV40和BKV T Ag。在总共20种SV40特异性肽和21种BKV特异性肽中，基于离子交换色谱法有7对相似的肽。这些共洗脱肽含有约25%至30%的总甲硫氨酸放射性。当将来自裂解感染细胞的SV40 T Ag的胰蛋白酶肽与来自病毒转化细胞的BKV T Ag的胰蛋白酶肽进行比较时，也获得了类似的结果。
