Rafsanjani Fatemeh N, Ardakani Zakieh V, Vahedian Jalal, Moradi Mehran, Attar Amir F
Tehran University of Medical Sciences and Health Services, Tehran, Iran.
Saudi J Gastroenterol. 2007 Oct-Dec;13(4):172-5. doi: 10.4103/1319-3767.36747.
BACKGROUND/AIMS: Paraoxon, an organophosphate metabolite of the insecticide parathion inhibits the enzyme, acetylcholinesterase (Achase). Organophosphates affect the heart, visual system, nervous system and muscles. In this present study, we investigate the effects of the chronic consumption of paraoxon on gastric acid and pepsin secretion in N-mari rats.
This study was performed from April 2003 to May 2004 in the Physiology department of Baghiatalah University of Medical Sciences, Tehran, Iran. It was performed on three groups of female N-mari rats (10 /group) each weighing 200-250 g. The first group received 0.05 mg/kg/day paraoxon subcutaneously for one month. The second group received the same chronic doses of ethyl alcohol (96%) (solvent of paraoxon) and the third group (control) received no drugs. After tracheostomy and laparatomy, gastric secretions were collected with a tube via the duodenum. Pentagastrin (25 microg/kg, i.p.) was used as a gastric stimulator. Acid and pepsin secretions were measured by titration and the Anson method, respectively. The stages of the measurements were basal (first and second), stimulated and returned-basal.
Basal acid secretion in the paraoxon group was greater than those in the alcohol and control groups (14.61 +/- 1.46, 7.18 +/- 0.28 and 7.88 +/- 0.26 micromol/15 min, respectively, P < 0.001)). Although pentagastrin-stimulated acid secretion in all the three groups was greater than that of the basal state, there were no significant differences among the three groups. Basal pepsin secretions in the paraoxon group were greater than those in the alcohol and control groups (2.97 +/- 0.32, 1.19 +/- 0.25 and 0.55 +/- 0.06 microg/15 min, respectively). Pentagastrin-stimulated pepsin secretion in the paraoxon group was significantly greater than those in the alcohol and control groups (3.22 +/- 0.38, 2.22 +/- 0.46 and 1.09 +/- 0.66 microg/15 min, respectively, P < 0.001).
Chronic exposure to paraoxon results in the increased secretion of gastric acid and pepsin.
背景/目的：对氧磷是杀虫剂对硫磷的有机磷酸酯代谢产物，可抑制乙酰胆碱酯酶（Achase）。有机磷酸酯会影响心脏、视觉系统、神经系统和肌肉。在本研究中，我们调查了长期摄入对氧磷对N - mari大鼠胃酸和胃蛋白酶分泌的影响。
本研究于2003年4月至2004年5月在伊朗德黑兰巴吉塔拉医科大学生理学系进行。实验对象为三组雌性N - mari大鼠（每组10只），每只体重200 - 250克。第一组大鼠皮下注射0.05毫克/千克/天的对氧磷，持续一个月。第二组大鼠接受相同慢性剂量的乙醇（96%）（对氧磷的溶剂），第三组（对照组）不给予任何药物。在进行气管切开术和剖腹术后，通过十二指肠用导管收集胃液。使用五肽胃泌素（25微克/千克，腹腔注射）作为胃刺激剂。分别通过滴定法和安森法测量胃酸和胃蛋白酶的分泌量。测量阶段包括基础期（第一和第二阶段）、刺激期和恢复基础期。
对氧磷组的基础胃酸分泌量高于乙醇组和对照组（分别为14.61±1.46、7.18±0.28和7.88±0.26微摩尔/15分钟，P<0.001）。尽管三组中五肽胃泌素刺激后的胃酸分泌量均高于基础状态，但三组之间无显著差异。对氧磷组的基础胃蛋白酶分泌量高于乙醇组和对照组（分别为2.97±0.32、1.19±0.25和0.55±0.06微克/15分钟）。对氧磷组中五肽胃泌素刺激后的胃蛋白酶分泌量显著高于乙醇组和对照组（分别为3.22±0.38、2.22±0.46和1.09±0.66微克/15分钟，P<0.001）。
长期接触对氧磷会导致胃酸和胃蛋白酶分泌增加。
