Nabavizadeh Rafsanjani Fatemeh, Vahedian Jalal
Kerman University of Medical Sciences and Health Services, Kerman, Iran.
Diabetes Res Clin Pract. 2004 Oct;66(1):1-6. doi: 10.1016/j.diabres.2004.02.017.
Diabetes mellitus is one of the most common endocrine diseases and affects most body organs. It affects gastric acid secretion, but this effect has not been fully understood. As the effects of diabetes on gastric pepsin secretion has not been proved yet, in this experimental study basal and distension-stimulated acid and pepsin secretions of diabetic and non-diabetic rats have been compared.
Female N-Mari rats weighing 200-250 g were used. Diabetic state was induced by intraperitoneal injection of 75 mg/kg streptozotocin. Animals were anaesthetized by the interaperitoneal injection of 60 mg/kg thiopental sodium. Then tracheostomy and laparotomy were done and gastric secretions were collected by a cannula entered via duodenum. Gastric distention induce by 1.5 ml normal saline per each 100g of body weight in stomach. Acid and pepsin were measured by titration and Anson's method, respectively.
Basal gastric secretions were similar in diabetic and non-diabetic animals. Distention-stimulated acid secretions in diabetic and non-diabetic rats were 3.24 +/- 0.16 and 8.05 +/- 0.21 micromol/15 min, respectively, which were significantly different (P = 0.00001). Distention-induced pepsin secretion in diabetic and non diabetic rats were 3.16 +/- 0.13 and 5.24 +/- 0.16 microg/15min, respectively, which were significantly different (P = 0.00001).
In this study the stomach of diabetic animals showed less reaction to distention, which may be due to the reduction of acid and pepsin secretary cells, reduction of the function of the cells, gastric atrophy or gastric vagus neuropathy. These probabilities need to be examined.
糖尿病是最常见的内分泌疾病之一，会影响身体的大多数器官。它会影响胃酸分泌，但这种影响尚未完全明确。由于糖尿病对胃蛋白酶分泌的影响尚未得到证实，因此在本实验研究中，对糖尿病大鼠和非糖尿病大鼠的基础胃酸分泌以及扩张刺激后的胃酸和胃蛋白酶分泌进行了比较。
选用体重200 - 250克的雌性N - Mari大鼠。通过腹腔注射75毫克/千克链脲佐菌素诱导糖尿病状态。通过腹腔注射60毫克/千克硫喷妥钠对动物进行麻醉。然后进行气管切开术和剖腹术，并通过经十二指肠插入的套管收集胃液。每100克体重向胃内注入1.5毫升生理盐水诱导胃扩张。分别通过滴定法和安森法测量胃酸和胃蛋白酶。
糖尿病大鼠和非糖尿病大鼠的基础胃液分泌相似。糖尿病大鼠和非糖尿病大鼠扩张刺激后的胃酸分泌分别为3.24±0.16和8.05±0.21微摩尔/15分钟，差异有统计学意义（P = 0.00001）。糖尿病大鼠和非糖尿病大鼠扩张诱导的胃蛋白酶分泌分别为3.16±0.13和5.24±0.16微克/15分钟，差异有统计学意义（P = 0.00001）。
在本研究中，糖尿病动物的胃对扩张的反应较小，这可能是由于胃酸和胃蛋白酶分泌细胞减少、细胞功能降低、胃萎缩或胃迷走神经病变。这些可能性需要进一步研究。
