含有磺化糖重复单元的聚合物刷：凝血激活的合成、表征及体外测试
Polymer Brushes Containing Sulfonated Sugar Repeat Units: Synthesis, Characterization and In Vitro Testing of Blood Coagulation Activation.
作者信息
Ayres N, Holt D J, Jones C F, Corum L E, Grainger D W
机构信息
Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, Utah 84112-5820 USA.
出版信息
J Polym Sci A Polym Chem. 2008;46(23):7713-7724. doi: 10.1002/pola.23075.
Abstract
A new polymer brush chemistry containing sulfonated carbohydrate repeat units has been synthesized from silicon substrates using ATRP methods and characterized both in bulk and using surface analysis. The polymer brush was designed to act as a mimic for the naturally occurring sulfonated glycosaminoglycan, heparin, commonly used for modifying blood-contacting surfaces both in vitro and in vivo. Surface analysis showed conversion of brush saccharide precursor chemistry to the desired sulfonated polymer product. The sulfonated polymer brush surface was further analyzed using three conventional in vitro tests for blood compatibility -- plasma recalcification times, complement activation, and thrombin generation. The sulfonated polymer brush films on silicon oxide wafers exhibited better assay performance in these blood component assays than the unsulfonated sugar functionalized polymer brush in all tests performed.
摘要
一种含有磺化碳水化合物重复单元的新型聚合物刷化学物质已通过原子转移自由基聚合（ATRP）方法从硅基底合成，并进行了本体表征和表面分析。该聚合物刷旨在模拟天然存在的磺化糖胺聚糖——肝素，肝素常用于在体外和体内修饰与血液接触的表面。表面分析表明，刷状糖前体化学物质已转化为所需的磺化聚合物产物。使用三种常规的血液相容性体外测试——血浆复钙时间、补体激活和凝血酶生成，对磺化聚合物刷表面进行了进一步分析。在所有进行的测试中，氧化硅晶片上的磺化聚合物刷膜在这些血液成分分析中表现出比未磺化的糖功能化聚合物刷更好的检测性能。
相似文献
1
Polymer Brushes Containing Sulfonated Sugar Repeat Units: Synthesis, Characterization and In Vitro Testing of Blood Coagulation Activation.含有磺化糖重复单元的聚合物刷：凝血激活的合成、表征及体外测试
J Polym Sci A Polym Chem. 2008;46(23):7713-7724. doi: 10.1002/pola.23075.
2
Erratum: Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification.勘误：用于蛋白质纯化的聚（丙烯酸五氟苯酯）功能化二氧化硅微珠的制备
J Vis Exp. 2019 Apr 30(146). doi: 10.3791/6328.
3
Bioactive zwitterionic polymer brushes grafted from silicon wafers via SI-ATRP for enhancement of antifouling properties and endothelial cell selectivity.通过 SI-ATRP 将生物活性两性离子聚合物刷从硅片上接枝，以提高抗污染性能和内皮细胞选择性。
J Biomater Sci Polym Ed. 2017 Dec;28(18):2101-2116. doi: 10.1080/09205063.2017.1376829. Epub 2017 Sep 19.
4
Polymer Brush Coating and Adhesion Technology at Scale.大规模聚合物刷涂层与粘附技术
Polymers (Basel). 2020 Jun 30;12(7):1475. doi: 10.3390/polym12071475.
5
Hydrophilic Aldehyde-Functional Polymer Brushes: Synthesis, Characterization, and Potential Bioapplications.亲水性醛基功能聚合物刷：合成、表征及潜在生物应用
Macromolecules. 2023 Feb 22;56(5):2070-2080. doi: 10.1021/acs.macromol.2c02471. eCollection 2023 Mar 14.
6
Influence of dynamic flow conditions on adsorbed plasma protein corona and surface-induced thrombus generation on antifouling brushes.动态流条件对抗污刷上吸附的血浆蛋白冠和表面诱导血栓形成的影响。
Biomaterials. 2018 Jun;166:79-95. doi: 10.1016/j.biomaterials.2018.03.009. Epub 2018 Mar 8.
7
Carbohydrate structure dependent hemocompatibility of biomimetic functional polymer brushes on surfaces.基于表面仿生功能聚合物刷的碳水化合物结构依赖性血液相容性。
Adv Healthc Mater. 2012 Mar;1(2):199-213. doi: 10.1002/adhm.201100042.
8
Antifouling Surfaces Based on Fluorine-Containing Asymmetric Polymer Brushes: Effect of Chain Length of Fluorinated Side Chain.基于含氟不对称聚合物刷的防污表面：氟化侧链链长的影响。
Langmuir. 2019 Feb 5;35(5):1235-1241. doi: 10.1021/acs.langmuir.8b03632. Epub 2018 Dec 24.
9
Light-Activated, Bioadhesive, Poly(2-hydroxyethyl methacrylate) Brush Coatings.光激活、生物黏附性、聚(2-羟乙基甲基丙烯酸酯)刷状涂层。
Biomacromolecules. 2020 Jan 13;21(1):240-249. doi: 10.1021/acs.biomac.9b01196. Epub 2019 Oct 23.
10
Facile method to prepare smooth and homogeneous polymer brush surfaces of varied brush thickness and grafting density.一种简便的方法可制备具有不同刷厚和接枝密度的光滑均一聚合物刷表面。
Langmuir. 2009 Dec 1;25(23):13448-55. doi: 10.1021/la901785t.
引用本文的文献
1
BMP-Binding Polysulfonate Brushes to Control Growth Factor Presentation and Regulate Matrix Remodelling.用于控制生长因子呈现和调节基质重塑的骨形态发生蛋白结合聚磺酸盐刷
ACS Appl Mater Interfaces. 2024 Aug 7;16(31):40455-40468. doi: 10.1021/acsami.4c05139. Epub 2024 Jul 29.
2
Synthesis of a series of benzothiazole amide derivatives and their biological evaluation as potent hemostatic agents.一系列苯并噻唑酰胺衍生物的合成及其作为有效止血剂的生物学评价。
RSC Adv. 2018 Feb 7;8(12):6231-6241. doi: 10.1039/c7ra13397a. eCollection 2018 Feb 6.
3
Nitric Oxide Release for Improving Performance of Implantable Chemical Sensors - A Review.用于改善可植入化学传感器性能的一氧化氮释放——综述
Appl Mater Today. 2017 Dec;9:589-597. doi: 10.1016/j.apmt.2017.10.002. Epub 2017 Nov 9.
4
Recent advances in thromboresistant and antimicrobial polymers for biomedical applications: just say yes to nitric oxide (NO).用于生物医学应用的抗血栓和抗菌聚合物的最新进展：对一氧化氮（NO）说“是”。
Biomater Sci. 2016 Aug 19;4(8):1161-83. doi: 10.1039/c6bm00271d. Epub 2016 May 26.
5
Strategies Toward Well-Defined Polymer Nanoparticles Inspired by Nature: Chemistry versus Versatility.受自然启发制备明确聚合物纳米颗粒的策略：化学与多功能性
J Polym Sci A Polym Chem. 2012 May 15;50(10):1869-1880. doi: 10.1002/pola.25955.
6
Synthesis and anticoagulant activity of polyureas containing sulfated carbohydrates.含硫酸化碳水化合物的聚脲的合成及其抗凝活性
Biomacromolecules. 2014 Dec 8;15(12):4455-66. doi: 10.1021/bm501245v. Epub 2014 Nov 5.
本文引用的文献
1
Polymeric brushes as functional templates for immobilizing ribonuclease A: study of binding kinetics and activity.作为固定核糖核酸酶A的功能模板的聚合物刷：结合动力学和活性研究
Langmuir. 2008 Feb 5;24(3):913-20. doi: 10.1021/la702510z. Epub 2007 Dec 13.
2
Covalent immobilization of antibody fragments on well-defined polymer brushes via site-directed method.通过定点方法将抗体片段共价固定在明确的聚合物刷上。
Colloids Surf B Biointerfaces. 2008 Apr 1;62(2):288-98. doi: 10.1016/j.colsurfb.2007.10.018. Epub 2007 Nov 4.
3
Complement activation on surfaces modified with ethylene glycol units.在由乙二醇单元修饰的表面上的补体激活。
Biomaterials. 2008 Feb;29(5):551-60. doi: 10.1016/j.biomaterials.2007.10.015. Epub 2007 Nov 5.
4
One-step surface modification of poly(lactide-co-glycolide) microparticles with heparin.聚（丙交酯-乙交酯）微粒与肝素的一步法表面改性
Pharm Res. 2007 Dec;24(12):2381-93. doi: 10.1007/s11095-007-9378-1. Epub 2007 Aug 21.
5
Polymeric coatings that mimic the endothelium: combining nitric oxide release with surface-bound active thrombomodulin and heparin.模拟内皮的聚合物涂层：将一氧化氮释放与表面结合的活性血栓调节蛋白和肝素相结合。
Biomaterials. 2007 Oct;28(28):4047-55. doi: 10.1016/j.biomaterials.2007.06.002. Epub 2007 Jun 26.
6
RGD-Functionalized polymer brushes as substrates for the integrin specific adhesion of human umbilical vein endothelial cells.RGD功能化聚合物刷作为人脐静脉内皮细胞整合素特异性黏附的底物
Biomaterials. 2007 Jun;28(16):2536-46. doi: 10.1016/j.biomaterials.2007.02.006. Epub 2007 Feb 15.
7
The influence of biomaterials on endothelial cell thrombogenicity.生物材料对内皮细胞血栓形成性的影响。
Biomaterials. 2007 Jun;28(16):2547-71. doi: 10.1016/j.biomaterials.2007.01.039. Epub 2007 Feb 9.
8
Plasma-deposited tetraglyme surfaces greatly reduce total blood protein adsorption, contact activation, platelet adhesion, platelet procoagulant activity, and in vitro thrombus deposition.等离子体沉积的四甘醇二甲醚表面能极大地降低全血蛋白吸附、接触激活、血小板黏附、血小板促凝活性及体外血栓形成。
J Biomed Mater Res A. 2007 Jun 15;81(4):827-37. doi: 10.1002/jbm.a.31091.
9
Relationship between blood compatibility and water structure--comparative study between 2-methoxyethylacrylate- and 2-methoxyethylmethacrylate-based random copolymers.血液相容性与水结构之间的关系——基于丙烯酸2-甲氧基乙酯和甲基丙烯酸2-甲氧基乙酯的无规共聚物的比较研究
J Biomed Mater Res A. 2007 Jun 1;81(3):710-9. doi: 10.1002/jbm.a.31113.
10
In vivo biocompatibility of sulfonated PEO-grafted polyurethanes for polymer heart valve and vascular graft.用于聚合物心脏瓣膜和血管移植物的磺化聚环氧乙烷接枝聚氨酯的体内生物相容性
Artif Organs. 2006 Dec;30(12):955-9. doi: 10.1111/j.1525-1594.2006.00327.x.
Zotero 插件