Garfias Yonathan, Bautista-De Lucio Víctor Manuel, García Cynthia, Nava Angel, Villalvazo Leonardo, Jiménez-Martínez María Carmen
Research Unit, Institute of Ophthalmology Conde de Valenciana Mexico City, Mexico.
Mol Vis. 2009 Oct 17;15:2068-73.
The pterygium is characterized by a fibrovascular neoformation from the bulbar conjunctiva into the cornea. The recent discovery that abnormal markers associated with tumor diseases are identified in the pterygium strengthens the theory that the pterygium is a tumor-like disease rather than a degenerative disease. The CD30 molecule has been identified in neoplastic and normal epithelial proliferating cells. The aim of this study was to determine the expression of the CD30 molecule in the pterygium.
Immunohistochemical staining using an antibody to CD30 and to the Ki-67 nuclear antigen was performed on 25 pterygial specimens and 10 healthy conjunctivas.
Strong immunostaining against CD30 was observed in all pterygium specimens, in contrast to the healthy conjunctivas that showed weak immono-positivity in only three cases. The staining was diffused, predominantly to the basal epithelium. The Ki-67 antigen was observed in the nucleus of the basal epithelium of the pterygial specimens, and no staining was observed in the healthy conjunctiva. When serial sections were stained with CD30 and Ki-67, the cells that expressed CD30 also expressed Ki-67.
The present study identified for the first time the existence of CD30 molecules in the basal epithelium of a pterygium. The fact that the positivity to Ki-67 in the basal epithelium of the pterygium correlated with the CD30 reactivity suggested that this protein could be associated with the uncontrolled cell proliferation of the epithelium in this pathology. The CD30 molecule could therefore be a suitable target to be inhibited using chimerical antibodies in pterygium diseases.
翼状胬肉的特征是从球结膜向角膜形成纤维血管新生组织。最近发现翼状胬肉中存在与肿瘤疾病相关的异常标志物，这强化了翼状胬肉是一种肿瘤样疾病而非退行性疾病的理论。CD30分子已在肿瘤性和正常上皮增殖细胞中被鉴定出来。本研究的目的是确定CD30分子在翼状胬肉中的表达。
对25例翼状胬肉标本和10例健康结膜进行免疫组织化学染色，使用抗CD30抗体和抗Ki-67核抗原抗体。
在所有翼状胬肉标本中均观察到针对CD30的强免疫染色，相比之下，健康结膜仅在3例中显示出弱阳性。染色呈弥漫性，主要位于基底上皮。在翼状胬肉标本的基底上皮细胞核中观察到Ki-67抗原，而在健康结膜中未观察到染色。当连续切片用CD30和Ki-67染色时，表达CD30的细胞也表达Ki-67。
本研究首次确定翼状胬肉基底上皮中存在CD30分子。翼状胬肉基底上皮中Ki-67阳性与CD反应性相关这一事实表明，该蛋白可能与这种病理状态下上皮细胞的失控增殖有关。因此，CD30分子可能是翼状胬肉疾病中使用嵌合抗体进行抑制的合适靶点。
