Zhang Li-Wei, Chen Bai-Hua, Xi Xing-Hua, Han Qian-Qian, Tang Luo-Sheng
Department of Ophthalmology, the Second Xiangya Hospital of Central South University, Changsha 410011, Hunan Province, China.
Int J Ophthalmol. 2011;4(4):388-92. doi: 10.3980/j.issn.2222-3959.2011.04.13. Epub 2011 Aug 18.
To assess the expression of anti-apoptotic protein survivin and tumor suppressor p53 protein in primary and recurrent pterygium and to investigate the relationship between them.
Survivin was assessed immunohistochemically using rabbit polyclonal antibody and p53 using mouse monoclonal antibody in a study sample of 20 cases of primary pterygium, 10 cases of recurrent pterygium and 10 cases of normal conjunctiva. RESLULTS: In our study, 35% of primary (7 of 20) and 40% of recurrent (4 of 10) pterygium specimens were positive for survivin staining; 45% of primary (9 of 20) and 50% of recurrent (5 of 10) pterygium specimens were positive for p53 expression; and all normal conjunctiva showed no staining of either survivin or p53. The p53 and survivin immunoreactivity in primary and recurrent pterygium groups was greater than those in normal conjunctiva group (P<0.05). There were no differences in p53 and survivin immunoreactivity between groups of primary and recurrent pterygium (P>0.05). The expression of survivin clearly segregated with p53-positive pterygium as compared with p53-negative cases [8 of 14 cases (57.1%) vs 3 of 16 cases (15.2%)]. The Fisher's exact test analysis confirmed a highly statistically significant correlation between survivin and p53 expression (P<0.05).
The survivin and p53 are overexpressed with correlation between them in primary and recurrent pterygium.
评估抗凋亡蛋白生存素和肿瘤抑制蛋白p53在原发性和复发性翼状胬肉中的表达情况，并探讨它们之间的关系。
采用兔多克隆抗体免疫组织化学法检测20例原发性翼状胬肉、10例复发性翼状胬肉和10例正常结膜标本中的生存素，采用鼠单克隆抗体检测p53。结果：在本研究中，35%的原发性翼状胬肉标本（20例中的7例）和40%的复发性翼状胬肉标本（10例中的4例）生存素染色呈阳性；45%的原发性翼状胬肉标本（20例中的9例）和50%的复发性翼状胬肉标本（10例中的5例）p53表达呈阳性；所有正常结膜标本生存素和p53均无染色。原发性和复发性翼状胬肉组的p53和生存素免疫反应性高于正常结膜组（P<0.05）。原发性和复发性翼状胬肉组之间的p53和生存素免疫反应性无差异（P>0.05）。与p53阴性病例相比，p53阳性翼状胬肉中生存素的表达明显不同[14例中的8例（57.1%）对16例中的3例（15.2%）]。费舍尔精确检验分析证实生存素和p53表达之间存在高度统计学显著相关性（P<0.05）。
原发性和复发性翼状胬肉中生存素和p53均过度表达且二者存在相关性。
