Howell B W, Afar D E, Lew J, Douville E M, Icely P L, Gray D A, Bell J C
Department of Biochemistry, McGill University, Montreal, Quebec, Canada.
Mol Cell Biol. 1991 Jan;11(1):568-72. doi: 10.1128/mcb.11.1.568-572.1991.
We have cloned a novel kinase (STY) from an embryonal carcinoma cell line. Sequence analysis of the STY cDNA reveals that it shares sequence homology with serine/threonine-type kinases and yet the bacterial expression product of the STY cDNA appears to have serine-, threonine-, and tyrosine-phosphorylating activities. The predicted STY protein is highly basic and contains a putative nuclear localization signal. During differentiation, two new mRNAs were detected in addition to the embryonic transcript.
我们从一个胚胎癌细胞系中克隆出了一种新型激酶（STY）。对STY cDNA的序列分析表明，它与丝氨酸/苏氨酸型激酶具有序列同源性，然而STY cDNA的细菌表达产物似乎具有丝氨酸、苏氨酸和酪氨酸磷酸化活性。预测的STY蛋白碱性很强，并含有一个假定的核定位信号。在分化过程中，除了胚胎转录本外，还检测到了两种新的mRNA。
