Duncan P I, Howell B W, Marius R M, Drmanic S, Douville E M, Bell J C
Ottawa Regional Cancer Centre, Cancer Research Group, Ontario, Canada.
J Biol Chem. 1995 Sep 15;270(37):21524-31. doi: 10.1074/jbc.270.37.21524.
The LAMMER subfamily of kinases has been conserved throughout evolution, and its members are thought to play important roles in the regulation of cellular growth and differentiation programs. STY is a murine LAMMER kinase which has been implicated in the control of PC12 cell differentiation. Multiple transcripts are derived from the Sty gene, and their relative abundance is developmentally regulated. Alternative splicing of the primary Sty transcript generates mRNAs encoding full-length catalytically active (STY) and truncated, kinase-deficient polypeptides. Both STY and its truncated isoform, STYT, are localized in the nucleus and are capable of heterodimerizing. We also demonstrate that STY functions as a dual specificity kinase in mammalian cells.
激酶的LAMMER亚家族在整个进化过程中一直保守，其成员被认为在细胞生长和分化程序的调节中发挥重要作用。STY是一种小鼠LAMMER激酶，与PC12细胞分化的控制有关。多个转录本源自Sty基因，它们的相对丰度受到发育调控。初级Sty转录本的可变剪接产生编码全长催化活性（STY）和截短的、激酶缺陷型多肽的mRNA。STY及其截短的异构体STYT都定位于细胞核中，并且能够形成异源二聚体。我们还证明STY在哺乳动物细胞中作为双特异性激酶发挥作用。
