Rivera Windell L, Yason John Anthony D L, Adao Davin Edric V
Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City, 1101, Philippines.
Primates. 2010 Jan;51(1):69-74. doi: 10.1007/s10329-009-0174-x. Epub 2009 Oct 28.
Entamoeba histolytica is a protozoan parasite that infects man and animals. This parasite has a global distribution and the disease it causes is usually characterized by diarrhea. In order to detect the parasite, it is necessary to differentiate it from Entamoeba dispar. E. dispar appears morphologically similar to E. histolytica but does not cause disease and tissue invasion. This study reports on the prevalence of E. histolytica and E. dispar among captive macaques in a primate facility in the Philippines. PCR was used to correctly identify both Entamoeba species. Indirect fluorescent antibody test (IFAT) was also performed to determine the seroprevalence of amebiasis in the captive macaques. Based on PCR targeting of the peroxiredoxin gene, of the 96 stool samples collected, 23 (24%) contained E. histolytica while 32 (33%) contained E. dispar. IFAT revealed 26 (27%) serum samples positive for antibodies against E. histolytica. Sequence analysis of the 18S rRNA gene showed that the 23 E. histolytica isolates were identical to human E. histolytica isolates deposited in the GenBank and not Entamoeba nuttalli as found in macaques in other recent reports. The Philippines is a major exporter of monkeys for biomedical research purposes, so screening animals before transporting them to other locations lessens the risk of spreading zoonoses to a wider area. This is the first report of the molecular detection of E. histolytica and E. dispar among macaques in the Philippines. This study complements the limited information available on the animal hosts of E. histolytica in the Philippines.
溶组织内阿米巴是一种感染人类和动物的原生动物寄生虫。这种寄生虫在全球范围内分布，其所引起的疾病通常以腹泻为特征。为了检测这种寄生虫，有必要将其与迪斯帕内阿米巴区分开来。迪斯帕内阿米巴在形态上与溶组织内阿米巴相似，但不会引起疾病和组织侵袭。本研究报告了菲律宾一家灵长类动物设施中圈养猕猴体内溶组织内阿米巴和迪斯帕内阿米巴的流行情况。采用聚合酶链反应（PCR）正确鉴定了这两种内阿米巴物种。还进行了间接荧光抗体试验（IFAT）以确定圈养猕猴中阿米巴病的血清流行率。基于对过氧化物还原酶基因的PCR检测，在收集的96份粪便样本中，23份（24%）含有溶组织内阿米巴，32份（33%）含有迪斯帕内阿米巴。IFAT显示26份（27%）血清样本针对溶组织内阿米巴抗体呈阳性。18S核糖体RNA基因的序列分析表明，23株溶组织内阿米巴分离株与保存在GenBank中的人类溶组织内阿米巴分离株相同，而非近期其他报告中在猕猴体内发现的纳氏内阿米巴。菲律宾是用于生物医学研究目的的猴子的主要出口国，因此在将动物运往其他地方之前进行筛查可降低人畜共患病传播到更广泛地区的风险。这是菲律宾猕猴体内溶组织内阿米巴和迪斯帕内阿米巴分子检测的首次报告。本研究补充了菲律宾关于溶组织内阿米巴动物宿主的有限信息。
