Division of Gene Technology, AlbaNova University Center, Stockholm, Sweden.
Electrophoresis. 2009 Nov;30(21):3691-5. doi: 10.1002/elps.200900273.
We report on the incorporation of the Visual DNA concept in a genotyping assay as a simple and straightforward detection tool. The principle of trapping streptavidin-coated superparamagnetic beads of micrometer size for visualization of genetic variances is used for PrASE-based detection of a panel of mutations in the severe and common genetic disorder of cystic fibrosis. The method allows a final investigation of genotypes by the naked eye and the output is easily documented using a regular hand-held device with an integrated digital camera. A number of samples were run through the assay, showing rapid and accurate detection using superparamagnetic beads and an off-the-shelf neodymium magnet. The assay emphasizes the power of Visual DNA and demonstrates the potential value of the method in future point-of-care tests.
我们报告了将 Visual DNA 概念纳入基因分型检测中作为一种简单直接的检测工具。该原理是利用捕获链霉亲和素包被的微米大小的超顺磁性珠来可视化遗传变异，用于基于 PrASE 的囊性纤维化严重和常见遗传疾病突变的检测。该方法允许通过肉眼进行最终的基因型调查，并且使用带有集成数字摄像头的常规手持设备可以轻松记录结果。该检测方法对多个样本进行了检测，结果显示使用超顺磁性珠和市售钕磁铁可以快速准确地进行检测。该检测方法强调了 Visual DNA 的强大功能，并展示了该方法在未来即时检测中的潜在价值。
