Departments of Marine Science, University of Otago, Dunedin, New Zealand.
Biomarkers. 2009 Nov;14(7):452-64. doi: 10.3109/13547500903123026.
Two novel, non-destructive assays were developed to evaluate contaminant-induced lipid peroxidation (thiobarbituric acid-reacting substances, TBARS, levels) and haem biosynthesis disruption (porphyrin excretion) in decapod crabs. A laboratory experiment was conducted whereby pie-crust crabs (Cancer novaezelandiae) were fed cockles (Austrovenus stutchburyi) collected from a contaminated and reference site and TBARS levels and porphyrin excretion determined using fluorometric analysis in urine samples. Pyrene metabolite levels were also measured in the same urine samples to assess polycyclic aromatic hydrocarbon (PAH) exposure. Contaminant-exposed crabs exhibited elevated urinary TBARS and porphyrin levels and a strong correlation was found between these two assays and the urinary pyrene metabolite concentrations. However, there was large within-treatment variability, which precluded a clear separation between the control and the impacted group. Nevertheless, consistency in the direction of the response shows that the biomarkers reflect pollutant levels and validates the use of these simple techniques from human medicine for environmental assessments.
两种新的非破坏性分析方法被开发出来，用于评估污染物诱导的脂质过氧化（硫代巴比妥酸反应物质，TBARS 水平）和血色素生物合成中断（卟啉排泄）在十足目甲壳动物中。进行了一项实验室实验，其中将馅饼壳蟹（Cancer novaezelandiae）喂食从污染和参照地点采集的贻贝（Austrovenus stutchburyi），并使用荧光分析方法测定尿液样品中的 TBARS 水平和卟啉排泄。同样在尿液样品中测量了芘代谢物水平，以评估多环芳烃（PAH）暴露情况。暴露于污染物的螃蟹表现出尿液中 TBARS 和卟啉水平升高，并且这两种分析方法与尿液中芘代谢物浓度之间存在很强的相关性。然而，在处理内存在很大的变异性，这使得无法在对照组和受影响组之间进行清晰的区分。尽管如此，反应方向的一致性表明生物标志物反映了污染物水平，并验证了这些来自人类医学的简单技术在环境评估中的应用。
