Hendrikse A, Blekkenhorst G
Department of Radiotherapy, University of Cape Town, South Africa.
Radiat Res. 1991 Jan;125(1):28-33.
The effect of exposing WHT mice bearing the Fib/T tumor to a low-oxygen environment (8, 10, and 15% oxygen) for 48 h or 72 h before irradiation was compared, using an in vitro colony-forming excision assay, to the effect obtained when mice were pretreated with air. The response of the Fib/T tumor to radiation delivered in air was improved both by a 48-h and by a 72-h exposure of the animals to 8, 10, and 15% oxygen. However, the greatest tumor sensitization was achieved when mice were kept in 8% oxygen for 48 h before irradiation. These results are interpreted and discussed in relation to increases in the 2,3-DPG concentration, which were shown to occur when mice were exposed to a reduced oxygen environment. The relative importance of two models proposed to explain these findings is assessed. If mice pretreated with air were irradiated in hyperbaric oxygen, a similar tumor response was observed compared to that when mice were exposed to 8% oxygen for 48 h and then irradiated in air.
利用体外集落形成切除试验，比较了将携带Fib/T肿瘤的WHT小鼠在照射前置于低氧环境（氧气浓度分别为8%、10%和15%）中48小时或72小时的效果与小鼠在空气中预处理后的效果。将动物置于8%、10%和15%的氧气中48小时和72小时，均可提高Fib/T肿瘤对空气中辐射的反应。然而，在照射前将小鼠置于8%氧气环境中48小时，可实现最大程度的肿瘤致敏。结合小鼠暴露于低氧环境时2,3-二磷酸甘油酸（2,3-DPG）浓度的增加，对这些结果进行了解释和讨论。评估了为解释这些发现而提出的两种模型的相对重要性。如果在空气中预处理的小鼠在高压氧中进行照射，与小鼠在8%氧气中暴露48小时然后在空气中照射相比，观察到了相似的肿瘤反应。
