Università di Firenze, Dipartimento di Sanità pubblica, Viale Morgagni 48, 50134 Firenze, Italy.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Dec 1;877(31):3931-6. doi: 10.1016/j.jchromb.2009.09.026. Epub 2009 Oct 8.
A qualitative and quantitative method for the analysis of drugs of abuse (cocaine and benzoylecgonine, opiates) and some stimulants in human hair was developed and validated. Hair samples were incubated with phosphate buffer (pH 5.0), chosen as the extraction medium, extracted with Bond Elut Certify cartridges and analyzed by LC-MS-MS and LC-MS(3) as confirmation for positive results. The method proved to be specific, accurate and precise across the calibration range (0.1-30 ng/mg) where good linearity was observed. Total extraction recovery, intra-assay accuracy and precision, limits of detection and limits of quantitation were estimated. The method was successfully applied to the analysis of hair samples collected from drug abusers and it was suitable for routine analytical applications in the Antidoping Laboratory of Public Health Laboratory.
建立并验证了一种定性和定量分析人发中滥用药物（可卡因和苯甲酰爱康宁、阿片类药物）和一些兴奋剂的方法。将头发样品与磷酸盐缓冲液（pH 5.0）孵育，选择该缓冲液作为提取介质，用 Bond Elut Certify 小柱提取，并用 LC-MS-MS 和 LC-MS(3) 进行分析，以确证阳性结果。该方法在整个校准范围内（0.1-30 ng/mg）表现出特异性、准确性和精密度，且线性良好。对总提取回收率、内标测定精密度和准确度、检测限和定量限进行了评估。该方法成功应用于从滥用药物者采集的头发样品分析，适用于公共卫生实验室兴奋剂检测实验室的常规分析应用。
