Key Laboratory of Child Development and Learning Science, Ministry of Education, Southeast University, Nanjing 210096, China.
Institute of Child Development and Education, Research Center of Learning Science, Southeast University, Nanjing 210096, China.
Molecules. 2021 Jan 19;26(2):516. doi: 10.3390/molecules26020516.
Long-term dependence of illicit drugs impairs the function of the hypothalamic-pituitary-adrenal (HPA) axis, which regulates the secretion of endogenous steroids, cortisol, and cortisone. Thus, the present study aimed to develop a sensitive method for simultaneous determination of the multiple illicit drugs and two steroids in hair to monitor the status of illicit drug exposure and the physiological and psychological health of drug addicts. The target analytes were extracted from hair by incubation with 1 mL methanol for 24 h at 40 °C and then determined with LC-APCI-MS/MS. The validated method showed acceptable linearity ( > 0.99) in the range of 1.25-250 pg/mg for cortisol and cortisone, 2.5-125 pg/mg for heroin, 2.5-1250 pg/mg for ketamine, 2.5-5000 pg/mg for methamphetamine (MAM), 2.5-250 pg/mg for 3, 4-methylenedioxymethamphetamine (MDMA), morphine, and 6-monoacetylmorphine (6-AM). Limits of quantification were 1.6, 1.2, 1.6, 1.0, 1.4, 0.3, 2.1, and 1.2 pg/mg for cortisol, cortisone, heroin, ketamine, MAM, MDMA, morphine, and 6-AM, respectively. Method recoveries were from 90-115% for all analytes. Inter-day and intra-day coefficients of variation were within 10%. Finally, this method was successfully applied to detect the aforementioned analytes in hair among female drug addicts who self-reported to be MAM abuser, heroin abuser, ketamine abuser, and abuser of mixture drugs of MAM and heroin. MAM abusers with current MAM use showed significantly higher concentrations of cortisol, MAM, and MDMA than controls with drug withdrawal.
长期依赖非法药物会损害下丘脑-垂体-肾上腺（HPA）轴的功能，该轴调节内源性类固醇皮质醇和皮质酮的分泌。因此，本研究旨在开发一种灵敏的方法，用于同时测定头发中的多种非法药物和两种类固醇，以监测非法药物暴露情况以及吸毒者的生理和心理健康。将目标分析物在 40°C 下用 1 mL 甲醇孵育 24 小时提取自头发中，然后用 LC-APCI-MS/MS 进行测定。该验证方法在皮质醇和皮质酮的 1.25-250 pg/mg、海洛因的 2.5-125 pg/mg、氯胺酮的 2.5-1250 pg/mg、甲基苯丙胺（MAM）的 2.5-5000 pg/mg、3,4-亚甲二氧基甲基苯丙胺（MDMA）的 2.5-250 pg/mg、吗啡和 6-单乙酰吗啡（6-AM）的 1.6、1.2、1.6、1.0、1.4、0.3、2.1 和 1.2 pg/mg 范围内表现出可接受的线性（ > 0.99）。定量限分别为皮质醇、皮质酮、海洛因、氯胺酮、MAM、MDMA、吗啡和 6-AM 的 1.6、1.2、1.6、1.0、1.4、0.3、2.1 和 1.2 pg/mg。所有分析物的方法回收率在 90-115%之间。日间和日内变异系数均在 10%以内。最后，该方法成功地应用于检测自报为 MAM 使用者、海洛因使用者、氯胺酮使用者和 MAM 与海洛因混合药物使用者的女性吸毒者头发中的上述分析物。当前使用 MAM 的 MAM 使用者的皮质醇、MAM 和 MDMA 浓度明显高于停药的对照者。
