Department of Bacteriology and Immunology, Harvard Medical School, Boston.
J Exp Med. 1963 Jun 1;117(6):1053-62. doi: 10.1084/jem.117.6.1053.
The in vitro anamnestic antibody response of popliteal lymph node fragments to additions of antigen closely resembles the in vivo anamnestic antibody response in its sensitivity to antigen, in the time course of antibody production, and in the sequence of appearance and the morphology of the antibody containing cells. Most of the cells responsible for antibody synthesis remain in the explant and do not migrate, although a few can be found in the outgrowing sheet of cells. The smallest concentration of bovine serum albumin which stimulates an anamnestic response in vitro is about 1 x 10(-9) gm/ml.
腘窝淋巴结碎片的体外回忆性抗体反应对添加的抗原非常敏感,类似于体内回忆性抗体反应,在抗体产生的时间过程中,以及抗体产生细胞的出现和形态上都非常相似。尽管在向外生长的细胞层中可以发现少数细胞,但负责抗体合成的大多数细胞仍留在培养物中,不会迁移。在体外刺激回忆反应的牛血清白蛋白的最小浓度约为 1 x 10(-9) gm/ml。