Jacobson E B, Thorbecke G J
J Exp Med. 1969 Aug 1;130(2):287-97. doi: 10.1084/jem.130.2.287.
Popliteal lymph nodes were obtained from rabbits 4 days to 9 months after a primary injection of diphtheria toxoid or bovine gamma-globulin into the footpad. The ability of cells from these nodes to proliferate upon reexposure to antigen in vitro was compared to the height of the secondary response produced by tissue fragments. In addition, a comparison was made between the responsiveness of draining and contralateral lymph nodes. While the secondary antibody response in vitro increased markedly with the time after immunization at which the lymph nodes were taken from the animals, the degree of proliferation induced by antigen was highest with cells from lymph nodes taken early after priming (peak day 7) and was very much lower with lymph node cells taken longer than 3 wk after priming. This striking difference between these two responses has been discussed. Contralateral lymph nodes were much inferior to draining nodes in their ability to give a secondary antibody response in vitro, and never gave a detectable proliferative response. This difference became less marked with time after priming, but could still be demonstrated after 4 months. These results suggest a concentration of primed cells in the lymphoid tissue draining the site of injection, and a slow release of these cells into the circulation, to be distributed to the remaining lymphoid tissue.
在将白喉类毒素或牛γ球蛋白初次注射到兔脚垫后4天至9个月，获取腘淋巴结。将这些淋巴结的细胞在体外再次接触抗原时增殖的能力与组织碎片产生的二次反应的强度进行比较。此外，还对引流淋巴结和对侧淋巴结的反应性进行了比较。虽然体外二次抗体反应随着从动物身上获取淋巴结的免疫后时间显著增加，但抗原诱导的增殖程度在初次免疫后早期（第7天达到峰值）获取的淋巴结细胞中最高，而在初次免疫后3周以上获取的淋巴结细胞中则低得多。已经讨论了这两种反应之间的显著差异。对侧淋巴结在体外产生二次抗体反应的能力远低于引流淋巴结，并且从未产生可检测到的增殖反应。这种差异在初次免疫后的时间里变得不那么明显，但在4个月后仍可显示出来。这些结果表明，在注射部位引流的淋巴组织中存在致敏细胞的聚集，并且这些细胞缓慢释放到循环中，以分布到其余的淋巴组织中。
