Morris Institute for Medical Research, Chicago.
J Exp Med. 1912 Sep 1;16(3):269-79. doi: 10.1084/jem.16.3.269.
This work proves that the proteolytic action of leucocytes is not a simple cleavage by one ferment. There are present two proteases, one acting in an alkaline medium and the other in an acid medium. This confirms Opie's work. In the leucocytes there was found in addition an ereptic ferment which is capable of acting in an acid or alkaline medium. Since this action is lost by heating to 70 degrees C., and by drying and keeping the leucocytes, it probably represents a ferment which acts on the digestion products of the two proteases. Analogy with other ferments in the body confirms this opinion.
这项工作证明,白细胞的蛋白水解作用不是一种简单的单一酶分解。这里存在两种蛋白酶,一种在碱性介质中起作用,另一种在酸性介质中起作用。这证实了 Opie 的工作。在白细胞中还发现了一种分解酶,它能够在酸性或碱性介质中起作用。由于这种作用在加热至 70°C 时丧失,并且在干燥和保存白细胞时丧失,因此它可能代表一种作用于两种蛋白酶的消化产物的酶。与体内其他酶的类比证实了这一观点。