一种从固定组织中获得活细胞悬液以及进行单个细胞培养的方法。
A METHOD FOR OBTAINING SUSPENSIONS OF LIVING CELLS FROM THE FIXED TISSUES, AND FOR THE PLATING OUT OF INDIVIDUAL CELLS.
机构信息
Laboratories of The Rockefeller Institute for Medical Research.
出版信息
J Exp Med. 1916 Apr 1;23(4):549-55. doi: 10.1084/jem.23.4.549.
Abstract
Individual, living, tissue cells can be obtained in suspension by digesting with trypsin the clot of growing tissue cultures. Under these circumstances the living cells assume a spherical form. When washed and plated in fresh plasma they put out processes and proliferate. After growth in the new plates has occurred the digestion and plating can be repeated. The limits of the method have not yet been reached. We are at work on a number of the problems which it has opened up.
摘要
将生长的组织培养物的凝块用胰蛋白酶消化，可以得到悬浮的单个、活体组织细胞。在这种情况下，活体细胞呈球形。将其洗涤并在新鲜血浆中铺板后，细胞会伸出突起并增殖。在新平板中生长后，可重复进行消化和铺板。该方法的局限性尚未达到。我们正在研究它所带来的一些问题。
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