Department of Animal Pathology of The Rockefeller Institute for Medical Research, Princeton, N. J.
J Exp Med. 1918 Sep 1;28(3):333-44. doi: 10.1084/jem.28.3.333.
A bacillus was found associated in pure culture with an extensive lobar bronchopneumonia in calves. It occurs in the exudate as a minute bacillus in small groups. In cultures it appears in three forms: as a bacillus, as a coccus-like endospore or arthrospore, and as a conglomerate Actinomyces-like flake or colony with peripheral clubs. The bacillar and coccoid forms occur on agar, the Actinomyces form in the condensation water of coagulated serum (horse). The coccoid form is probably a spore state, the minute refringent spore being contained in a roundish, unstainable mass representing either the remnants of bacillar substance or some capsular material. The somewhat striking similarities between this organism and Actinomyces are expressed by the massed growth with terminal clubs, the bacillar and coccoid stages, all of which are characteristic of Actinomyces. Sealing the tubes is essential for multiplication. Cultures must be renewed within a few days, otherwise multiplication fails. The substance which forms the bulk of the radiate flocculi is probably of capsular nature, greatly overproduced in serum tubes and scarce or absent on agar. Its nature is unknown. The organism is not appreciably pathogenic when injected into certain small laboratory animals.
从牛的大叶性支气管肺炎的渗出物中，分离出一种与肺炎有关的杆菌，可在纯培养中生长。这种杆菌以小群形式出现在渗出物中，为小杆菌。在培养物中，它以三种形式出现：杆菌、球菌样芽孢或节孢子，以及类似于聚集的放线菌的片状或团块状菌落，周围有棒状结构。杆菌和球菌形式出现在琼脂上，放线菌形式出现在凝固血清（马）的冷凝水中。球菌形式可能是一种孢子状态，微小的折光孢子包含在圆形、不可染色的团块中，该团块代表杆菌物质的残余物或一些荚膜物质。该生物体与放线菌之间存在一些显著的相似之处，例如末端棒状聚集生长、杆菌和球菌阶段，所有这些都是放线菌的特征。密封试管对于增殖是必不可少的。培养物必须在几天内更新，否则增殖将失败。形成辐射状絮状物的大部分物质可能具有荚膜性质，在血清管中大量产生，在琼脂上则很少或不存在。其性质尚不清楚。该生物体在注射到某些小型实验动物中时，其致病性并不明显。
