Department of Animal Pathology of The Rockefeller Institute for Medical Research, Princeton, N. J.
J Exp Med. 1927 Feb 28;45(3):519-28. doi: 10.1084/jem.45.3.519.
A disease of cows characterized by swelling of the vulva, acute inflammation of the vaginal mucosa, accompanied by a more or less profuse mucopurulent exudate is described. After the acute inflammation subsides the mucosa becomes studded with tiny, round, raised, red nodules which persist 2 or 3 months or longer. The acute lesion consists in necrosis of the epithelium and accumulations of leucocytes and round cells in the edematous submucosa. The nodules in the later stages are made up of densely packed masses of lymphocytes in the submucosa which force the epithelial layer outward. A Gram-negative bacillus with tiny polar granules was found in the exudate. It measures I to 2micro in length and stains with difficulty. The organism was obtained in pure culture by inoculating the exudate into tubes of slanted agar to which defibrinated horse blood had been added. Growth occurs only in sealed tubes. The organism possesses slight pathogenicity for guinea pigs. When freshly isolated cultures were introduced into the vagina of heifers or young calves, acute inflammation resulted which terminated in the characteristic granular stage of the disease.
描述了一种以牛的外阴肿胀、阴道黏膜急性炎症为特征的疾病，伴有或多或少的脓性分泌物。急性炎症消退后，黏膜上出现许多小而圆、凸起、红色的小结节，持续 2 到 3 个月或更长时间。急性病变包括上皮坏死和水肿的黏膜下层中白细胞和圆形细胞的积聚。后期的结节由黏膜下层中密集排列的淋巴细胞组成，这些淋巴细胞将上皮层向外推。在渗出物中发现了一种带有微小极性颗粒的革兰氏阴性杆菌。它长 1 到 2 微米，染色困难。通过将渗出物接种到添加了去纤维马血的倾斜琼脂管中，可以获得纯培养物。只有在密封的管中才能生长。该生物体对豚鼠具有轻微的致病性。当新鲜分离的培养物被引入母牛或小牛的阴道时，会导致急性炎症，最终导致疾病的特征性颗粒阶段。
