二氧化碳对脑膜炎奈瑟菌培养的影响。
THE EFFECT OF CARBON DIOXIDE IN THE CULTIVATION OF THE MENINGOCOCCUS.
机构信息
Laboratories of The Rockefeller Institute for Medical Research.
出版信息
J Exp Med. 1919 Apr 1;29(4):321-8. doi: 10.1084/jem.29.4.321.
The meningococcus is not a "microaerophile." It grows equally well in atmospheres containing from 15 to 40 per cent oxygen. If small amounts of carbon dioxide affect the growth of the meningococcus on an artificial medium it is by changing the reaction of the medium, not by slightly reducing the oxygen tension of the surrounding air. The fallibility of titrating the total acidity of a medium is again clearly demonstrated. A reaction favorable to the meningococcus cannot be determined from the total titratable acidity but depends solely upon the hydrogen ion concentration of the medium. The optimum for the meningococcus is approximately at pH 7.4. The value of a moist chamber in the cultivation of the meningococcus is shown by unusually luxuriant growth when other conditions are also favorable.
脑膜炎球菌不是“微需氧菌”。它在含氧量为 15%至 40%的环境中同样能良好生长。如果少量的二氧化碳会影响人工培养基中脑膜炎球菌的生长，那也是通过改变培养基的反应，而不是通过稍微降低周围空气的氧气张力。这再次清楚地表明了滴定培养基总酸度的不可靠性。有利于脑膜炎球菌的反应不能仅从总可滴定酸度来确定，而完全取决于培养基中的氢离子浓度。脑膜炎球菌的最佳 pH 值约为 7.4。在其他条件也有利的情况下，潮湿室在培养脑膜炎球菌中的价值表现为异常旺盛的生长。
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