Anesthesia Laboratory of the Harvard Medical School at the Massachusetts General Hospital, Boston.
J Gen Physiol. 1944 Mar 20;27(4):325-38. doi: 10.1085/jgp.27.4.325.
Manometric measurements were made of oxygen uptake (Q(O(O2) )) and aerobic lactic acid output (Q(G)) by slices of cerebral cortex and medulla oblongata of the cat in the presence of mixtures of 1, 5, and 20 volumes per cent of carbon dioxide in oxygen. The concentrations of NaHCO(3) and NaCl in the medium were varied to maintain constant pH and sodium ion concentrations. The calcium ion concentration was 0.0002 M. At pH 7.5 under these conditions, an increase in carbon dioxide from 1 per cent to 5 per cent doubled the Q(G) of both tissues but did not alter Q(O(O2) ); an increase from 5 per cent to 20 per cent carbon dioxide had no further effect on Q(G) in either tissue or Q(O(O2) ) of cortex, but did depress the Q(O(O2) ) of medulla. At pH 8.1, an increase in carbon dioxide from 1 per cent to 5 per cent raised the Q(O(O2) ) and Q(G) of cortex by about 60 per cent. Measurements at low oxygen tension carried out previously in phosphate medium were repeated in bicarbonate medium to obtain data for the combined output of lactic acid and carbon dioxide (Q(A)). When the oxygen in the gas phase was decreased from 95 to 3 volumes per cent, the lactic acid output as measured colorimetrically increased by 114 mg./gm. in cortex and by 8 mg./gm. in medulla; Q(A) increased from 12.3 to 13.5 in cortex and decreased from 5.1 to 3.8 in medulla.
在含有 1、5 和 20 体积%二氧化碳的混合氧中，对猫大脑皮层和延髓切片进行了氧摄取 (Q(O2)) 和有氧乳酸输出 (Q(G)) 的测压测量。 介质中 NaHCO3 和 NaCl 的浓度变化以保持恒定的 pH 值和钠离子浓度。钙离子浓度为 0.0002M。在这些条件下 pH 为 7.5 时，二氧化碳从 1%增加到 5%使两种组织的 Q(G)增加了一倍，但 Q(O2) 没有变化；从 5%增加到 20%的二氧化碳对两种组织的 Q(G)或皮层的 Q(O2) 没有进一步影响，但降低了延髓的 Q(O2)。在 pH 8.1 时，二氧化碳从 1%增加到 5%使皮层的 Q(O2) 和 Q(G)增加了约 60%。以前在磷酸盐介质中进行的低氧张力测量在碳酸氢盐介质中重复进行，以获得乳酸和二氧化碳 (Q(A)) 的综合输出数据。当气相中的氧气从 95 减少到 3 体积%时，色测定法测量的乳酸输出在皮层中增加了 114mg/gm.，在延髓中增加了 8mg/gm.；Q(A)在皮层中从 12.3 增加到 13.5，在延髓中从 5.1 减少到 3.8。
