肺炎球菌酶的研究：二、脂肪酶：酯酶。
STUDIES ON THE ENZYMES OF PNEUMOCOCCUS : II. LIPOLYTIC ENZYMES: ESTERASE.
机构信息
Hospital of The Rockefeller Institute for Medical Research.
出版信息
J Exp Med. 1920 Oct 31;32(5):571-82. doi: 10.1084/jem.32.5.571.
Abstract
- Pneumococci contain an intracellular enzyme of marked lipolytic activity as measured by the acid liberated by its action on tributyrin. 2. Enzyme-containing solutions may be prepared by dissolving pneumococci in bile, or by extraction by other means. 3. The optimum reaction for maximum activity of the endolipase is about pH 7.8, which coincides with the optimum hydrogen ion concentration for growth of pneumococci. 4. Heating the enzyme for 10 minutes at 70 degrees C. destroys its activity. 5. Attenuation of virulence of pneumococcus had no measureable effect on enzyme activity. 6. The possible relation of the endolipase to the mechanism of bile solubility is discussed.
摘要
肺炎球菌含有一种细胞内脂酶，其脂解活性显著，可通过其对三丁酸酯的作用释放的酸来衡量。
可通过将肺炎球菌溶解在胆汁中或通过其他方法提取来制备含酶溶液。
内切脂酶最大活性的最佳反应 pH 值约为 7.8，与肺炎球菌生长的最佳氢离子浓度相吻合。
将酶在 70°C 下加热 10 分钟会使其失活。
肺炎球菌毒力的衰减对酶活性没有可衡量的影响。
讨论了内切脂酶与胆汁溶解度机制之间的可能关系。
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