Laboratories of The Rockefeller Institute for Medical Research.
J Exp Med. 1921 Jan 31;33(2):125-45. doi: 10.1084/jem.33.2.125.
An active substance has been detected, by the methods described, in five patients in early stages of epidemic influenza during 1918-19 and two patients in early stages of epidemic influenza during 1920 It was not detected in twelve cases of the same disease in which the onset of obvious symptoms occurred more than 36 hours before washing of the nasopharynx was carried out, nor was it found in the secretions of fourteen individuals free from the syndrome of influenza either during the epidemics or the interval between them. With this substance a clinical and pathological condition has been induced in rabbits, affecting the blood and pulmonary structures mainly, which could be maintained and carried through at least fifteen successive animals. For this reason, and also because of the dilution between passages, we are led to believe that we were dealing with the actual transmission of a multiplying agent rather than with a passive transference of an original active substance. In some of the experiments secondary infections by ordinary bacteria were encountered. The relation of these microorganisms to this active substance will be dealt with fully in another communication. However, the essential effects were produced by a substance wholly unrelated to these bacteria. The similarity that exists between the effects produced in rabbits on the blood and the lungs and those occurring in man in epidemic influenza provides a basis for further investigation on the inciting agent of epidemic influenza.
应用所述方法，在 1918-19 年流感大流行早期的 5 例患者和 1920 年流感大流行早期的 2 例患者中检测到一种活性物质。在 12 例同样疾病的患者中，明显症状出现前超过 36 小时进行鼻咽冲洗，未检测到该活性物质，在流感综合征流行期间或流行间歇期也未在 14 例无流感症状个体的分泌物中检测到该活性物质。用这种物质，诱发了家兔的一种临床和病理状况，主要影响血液和肺结构，至少可在 15 只连续的动物中维持和传递。基于此原因，也由于传代过程中的稀释，我们认为我们正在处理的是增殖剂的实际传播，而不是原始活性物质的被动转移。在一些实验中，遇到了普通细菌的继发感染。这些微生物与这种活性物质的关系将在另一份通讯中全面讨论。然而，完全不相关的细菌产生了这些基本的影响。在兔子血液和肺部产生的效应与人类流感大流行中出现的效应之间存在相似性，为进一步研究流感大流行的引发剂提供了依据。
