Association of and with Periodontitis Disease Severity and Red Complex Bacteria.

Oral biofilms are considered the principal etiological agent in the development of periodontitis. Novel species that may contribute to periodontitis and dysbiosis have been identified recently. The study aims to evaluate the presence of and in healthy and diseased patients and their association with clinical parameters and with red complex bacteria. The study included 60 subjects, with 30 patients each in the healthy and periodontitis groups. The clinical parameters were noted, and samples were subjected to DNA extraction followed by a polymerase chain reaction. Statistical analysis was performed using the Graph Pad Prism software. Results: and were detected at a significantly higher percentage in the periodontitis group compared to the healthy group ( < 0.05). was significantly associated with in the periodontitis group ( < 0.05). Both of these organisms were present in sites with higher clinical attachment loss ( < 0.05). This study demonstrated that both and were detected at a significantly higher percentage in periodontitis subjects and were detected more frequently in sites with a greater clinical attachment loss. It was also evident that both and can be present independently of other putative periodontal pathogens.