Laboratories of The Rockefeller Institute for Medical Research.
J Exp Med. 1922 Jan 1;35(1):63-76. doi: 10.1084/jem.35.1.63.
The flocculation of bacteria which have absorbed specific agglutinins may be mechanically effected by means of the centrifuge, with results that coincide with those obtained by the standard method of test. Specific serological differences between meningococci, for example, may be determined by the centrifuge method. The technique is described. By the elimination of the inconstant time factor in the flocculation phase opportunity is given for a closer analysis of specific absorption, and of the influence of various conditions upon both phases of agglutination. The velocity of the absorption reaction is a function of the temperature at which it occurs, and the acceleration with increasing temperature is of the order of chemical phenomena. The absorption reaction proceeds most rapidly near the temperature of antibody destruction. The injurious effect of high temperature is revealed first in the serum; the antigen-antibody complex is not less sensitive. The flocculation phase is also promoted by higher temperature, but lags far behind absorption, and consumes most of the time required for spontaneous agglutination. The presence of an excess of antibodies greatly accelerates absorption and flocculation. The absorption reaction, under such circumstances, is ordinarily completed within a relatively short time.
已吸收特定凝集素的细菌的絮凝作用可以通过离心来机械地实现，其结果与标准测试方法获得的结果一致。例如，脑膜炎球菌之间的特定血清学差异可以通过离心方法来确定。本文介绍了该技术。通过消除絮凝阶段中不定时因素，有机会更紧密地分析特定的吸收以及各种条件对凝集的两个阶段的影响。吸收反应的速度是发生温度的函数，随着温度的升高而加速，其顺序与化学现象相当。吸收反应在接近抗体破坏温度时最快。高温的有害影响首先在血清中显现，抗原-抗体复合物的敏感性并不降低。较高的温度也会促进絮凝阶段，但远远落后于吸收阶段，并且消耗了自发凝集所需的大部分时间。抗体过量的存在极大地加速了吸收和絮凝作用。在这种情况下，吸收反应通常在相对较短的时间内完成。
