Laboratories of The Rockefeller Institute for Medical Research.
J Exp Med. 1928 Jul 31;48(2):199-205. doi: 10.1084/jem.48.2.199.
Cultures of microorganisms similar to those described by Evans have been obtained in media inoculated with suspensions of herpes virus-infected brains prepared by grinding. But they have also been isolated from saline suspensions of uninoculated meat particles ground in a sterile mortar, and from dextrose broth treated in the same way. It is believed that these organisms are contaminants introduced during the process of grinding. Since they enter the material in no great number, one may suppose them to be suppressed by animals inoculated with the ground substance. In artificial media, on the other hand, they find favorable conditions for multiplication. In our experience, no growth of microorganisms is obtained in routine cultures of virus-infected brains, when fragments, instead of ground material, are used-a fact which may be taken to support the explanation just given. The tests of the part played by streptococci in experimental virus encephalitis failed to disclose that the microorganisms have any etiological relationship to the affection. The intracerebral injection of rabbits with the cultures procured in the course of the experiments produces a purulent type of meningoencephalitis which does not resemble virus encephalitis either in its symptom-complex or in its pathology. The same type of meningitis follows the injection of streptococci derived from ground meat particles, from "ground" broth, from normal brains, and those infected with herpes virus. Some rabbits manifested resistance to the streptococci, whereas all that have been inoculated intracerebrally with the three strains of herpes virus used in this study have proved susceptible thereto. Certain of the rabbits just mentioned which had proved resistant to streptococci inoculated into the brain or cornea were injected with herpes virus and reacted typically. Comparative tests have revealed that the streptococci are more sensitive to the destructive effect of 50 per cent glycerol than is herpes virus. From all this, it can be concluded that streptococci are not the visible form of herpes virus, nor do they produce in rabbits effects like those induced in the brain and cornea by the herpes virus.
类似 Evans 所描述的微生物培养物已经从通过研磨制备的感染疱疹病毒的脑的悬浮液接种的培养基中获得。但是，它们也从无菌研钵中研磨的未接种肉颗粒的盐水中悬浮液以及以相同方式处理的葡萄糖肉汤中分离出来。人们认为这些生物体是在研磨过程中引入的污染物。由于它们进入材料的数量不多，因此可以假设它们被接种了研磨物质的动物所抑制。另一方面，在人工培养基中，它们找到了繁殖的有利条件。根据我们的经验，当使用片段而不是研磨材料时，在常规感染病毒的脑培养物中不会获得微生物的生长-这一事实可以支持刚才给出的解释。链球菌在实验性病毒脑炎中所起作用的测试未能揭示出这些微生物与该疾病有任何病因关系。用在实验过程中获得的培养物对兔子进行脑内注射会导致化脓性脑膜脑炎，该脑炎在其症状复合体或病理学上均与病毒脑炎不同。从研磨肉颗粒，“研磨”肉汤，正常脑和感染疱疹病毒的脑中获得的链球菌注射也会引起相同类型的脑膜炎。某些兔子对链球菌具有抵抗力，而所有用本研究中使用的三种疱疹病毒株脑内接种的兔子都对其敏感。上述某些已证明对脑内或角膜内接种的链球菌具有抵抗力的兔子被注射了疱疹病毒，并且反应典型。比较测试表明，链球菌对 50％甘油的破坏作用比疱疹病毒更为敏感。由此可以得出结论，链球菌不是疱疹病毒的可见形式，也不会在兔子体内产生类似于疱疹病毒在脑和角膜中引起的作用。
