鸡肉瘤（鸡肿瘤 I-rous）滤液的氧化作用。
THE EFFECT OF OXIDATION OF FILTRATES OF A CHICKEN SARCOMA (CHICKEN TUMOR I-ROUS).
机构信息
Department of Bacteriology and Immunology, Harvard University Medical School, Boston.
出版信息
J Exp Med. 1928 Aug 31;48(3):343-9. doi: 10.1084/jem.48.3.343.
Abstract
- The rapid autoinactivation at 37 degrees of candle filtrates of the Rous Foul Sarcoma I is due in large part to oxidation. 2. Such oxidation may be prevented by a dilution of 1-2000 cysteine hydrochloride brought to pH 7.4 with NaOH. 3. The variability of infectiousness of candle filtrates is due in part at least to oxidative changes which take place during their preparation; and more active extracts may be obtained by preventing these by means of cysteine. 4. For elaborate experiments it is possible to preserve filtrates in the ice box with cysteine and vaseline until test inoculations have established their infectivity.
摘要
劳斯肉瘤 I 的烛形滤过液在 37 度时迅速自动失活，主要是由于氧化作用。
用氢氧化钠将 1-2000 盐酸半胱氨酸稀释至 pH7.4 可防止这种氧化作用。
烛形滤过液的感染力的变化至少部分是由于在制备过程中发生的氧化变化所致；通过半胱氨酸防止这种变化，可以得到更活跃的提取物。
为了进行精细的实验，可以在冰盒中用半胱氨酸和凡士林保存滤过液，直到试验接种确定其感染力。
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