Department of Animal Pathology of The Rockefeller Institute for Medical Research, Princeton, N. J.
J Exp Med. 1930 Apr 30;51(5):787-806. doi: 10.1084/jem.51.5.787.
Some media are described which inhibit bacterial growth, but are favorable to protozoan development. A purification technique, which takes advantage of geotropic responses, was devised and used successfully with 7 species of protozoa, including flagellates and ciliates. The method was also used with a Spirillum. For one flagellate which could not be purified in this manner, a procedure involving chemical sterilization was employed. Paramecium caudatum was purified, but failed to develop subsequently in the absence of living microorganisms. Four of the protozoa which were purified ingest other microorganisms normally. The work shows that purified protozoa grow well under proper conditions, and then they can be studied culturally and biologically, like bacteria.
有些媒体被描述为抑制细菌生长，但有利于原生动物的发展。一种利用向地性反应的纯化技术被设计出来，并成功地用于 7 种原生动物，包括鞭毛虫和纤毛虫。该方法也用于螺旋菌。对于一种不能用这种方法纯化的鞭毛虫，采用了化学灭菌的方法。尾草履虫被纯化，但在没有活微生物的情况下随后无法发育。在纯化的 4 种原生动物中，有 4 种可以正常摄取其他微生物。这项工作表明，在适当的条件下，纯化的原生动物生长良好，然后它们可以像细菌一样在文化和生物学上进行研究。
