一种用于纤毛虫及其他原生动物接种和克隆的简单方法。

A simple method for plating and cloning ciliates and other protozoa.

作者信息

Soldo A T, Brickson S A

出版信息

J Protozool. 1980 Aug;27(3):328-31. doi: 10.1111/j.1550-7408.1980.tb04271.x.

DOI:10.1111/j.1550-7408.1980.tb04271.x

Abstract

A simple method is described for plating and cloning ciliates and other protozoa, based on a principle differing from that traditionally used for plating and cloning bacteria and other microorganisms. This procedure, referred to as te silicone-oil-plating-procedure (SOPP), involves vortexing small volumes of culture medium containing protozoa with larger volumes of a non-toxic silicone oil and plating the resulting unstable emulsion in small plastic petri plates. Discrete microdroplets of culture medium form containing protozoa entrapped and immobilized between the hydrophobic surfaces of teh plastic petri dish and the oil. Protozoa, isolated by this method grow, divide, and multiply to form clones. The procedure may be used for plating and cloning protozoa in bacterized and axenic culture. Variations of te basic method may be applied to isolating protozoa from the wild, washing protozoa to remove microorganisms, screening for potential mutants, and for replica plating.

摘要

本文描述了一种用于纤毛虫和其他原生动物接种和克隆的简单方法，其原理不同于传统用于细菌和其他微生物接种和克隆的方法。该程序称为硅油接种程序（SOPP），包括将含有原生动物的少量培养基与大量无毒硅油涡旋混合，然后将所得不稳定乳液接种到小塑料培养皿中。含有原生动物的离散微滴形成，原生动物被困在塑料培养皿和油的疏水表面之间并固定。通过这种方法分离的原生动物生长、分裂并繁殖形成克隆。该程序可用于在细菌化和无菌培养中对原生动物进行接种和克隆。基本方法的变体可用于从野外分离原生动物、清洗原生动物以去除微生物、筛选潜在突变体以及用于影印接种。