Laboratories of The Rockefeller Institute for Medical Research.
J Exp Med. 1932 Apr 30;55(5):781-96. doi: 10.1084/jem.55.5.781.
With the idea that studies on the serological properties of peptides may ultimately aid in the understanding of the precipitin reactions of proteins, antigens have been prepared containing aminobenzoylated dipeptides, namely glycyl-glycine, glycyl-d, l-leucine, d, l-leucyl-glycine and d, l-leucyl-d, l-leucine. These four antigens were found to be different serologically, their specificity depending on the structure of the terminal amino acid carrying the free carboxyl group, and to a less degree also on the second amino acid. The results were obtained by means of precipitin and inhibition tests. Analogies to observations on enzyme specificity are discussed.
受这样一种观点的启发,即对肽的血清学性质进行研究最终可能有助于理解蛋白质的沉淀反应,我们制备了含有氨苯甲酰化二肽的抗原,即甘氨酰-甘氨酸、甘氨酰-DL-亮氨酸、DL-亮氨酰-甘氨酸和 DL-亮氨酰-DL-亮氨酸。这四种抗原在血清学上被发现是不同的,它们的特异性取决于携带游离羧基的末端氨基酸的结构,在较小程度上也取决于第二个氨基酸。这些结果是通过沉淀和抑制试验获得的。讨论了与酶特异性观察的类比。