DEODHAR S D
J Exp Med. 1960 Mar 1;111(3):429-39. doi: 10.1084/jem.111.3.429.
The parenteral administration of benzoylangiotensin II-azo-BGG to rabbits produced an antiserum with antiangiotensin activity. Antiangiotensin inhibited the biological action of p-aminobenzoylangiotensin II, valine(5)-angiotensin II (free acid form), isoleucine(5)-angiotensin II and valine(5)-angiotensin II (amide form), but it was totally inert towards angiotensin 1. Antiangiotensin activity was distinguished from that of serum angiotensinase by the following observations: (a) an angiotensinase-free gamma-globulin fraction contained antiangiotensin, (b) angiotensinase inactivated angiotensin II (both the amide and free acid forms) and angiotensin I in contrast to the remarkable specificity for angiotensin II exhibited by antiangiotensin. Serological demonstration of antiangiotensin included: (a) a comparison of its precipitin reaction with the angiotensin BGG complex with the reaction with BGG alone, (b) the partial inhibition of the precipitin reaction with the angiotensin BGG complex by angiotensin II, (c) a precipitin reaction with a different angiotensin II protein complex (cat serum). Angiotensin II administered parenterally as the free polypeptide was not antigenic.
给家兔肠胃外注射苯甲酰血管紧张素II - 偶氮 - 牛血清白蛋白产生了具有抗血管紧张素活性的抗血清。抗血管紧张素抑制了对氨基苯甲酰血管紧张素II、缬氨酸(5) - 血管紧张素II(游离酸形式)、异亮氨酸(5) - 血管紧张素II和缬氨酸(5) - 血管紧张素II(酰胺形式)的生物作用,但对血管紧张素I完全无活性。通过以下观察可将抗血管紧张素活性与血清血管紧张素酶的活性区分开来:(a)不含血管紧张素酶的γ - 球蛋白部分含有抗血管紧张素,(b)血管紧张素酶使血管紧张素II(酰胺和游离酸形式)和血管紧张素I失活,这与抗血管紧张素对血管紧张素II表现出的显著特异性形成对比。抗血管紧张素的血清学证明包括:(a)将其与血管紧张素 - 牛血清白蛋白复合物的沉淀反应与单独与牛血清白蛋白的反应进行比较,(b)血管紧张素II对血管紧张素 - 牛血清白蛋白复合物沉淀反应的部分抑制,(c)与不同的血管紧张素II蛋白质复合物(猫血清)的沉淀反应。以游离多肽形式肠胃外给药的血管紧张素II无抗原性。