在单个培养瓶中长时间培养组织。
THE CULTIVATION OF TISSUES FOR PROLONGED PERIODS IN SINGLE FLASKS.
机构信息
Laboratories of The Rockefeller Institute for Medical Research.
出版信息
J Exp Med. 1936 Jun 30;64(1):121-30. doi: 10.1084/jem.64.1.121.
Abstract
- Fragments of breast muscle from a 12 day old chick embryo have been kept alive in single flasks for an entire year without being transferred. The nutrient materials were supplied by frequent applications of adult fowl serum diluted with Tyrode solution. 2. When fragments of fixed tissues are cultivated in serum, cell multiplication and cell death are both reduced to an extremely low level. 3. The presence of a plasma coagulum is not essential to the continued survival and further development of tissues cultivated inserum. 4. The fibrinogen, prothrombin, and fibrin of coagulated plasma are not essential to the development of connective tissue fibers in vitro.
摘要
从 12 天大的鸡胚的胸肌组织中分离出来的组织碎片，在没有转移的情况下，在单个培养瓶中存活了整整一年。营养物质是通过频繁地应用用 Tyrode 溶液稀释的成鸡血清来供给的。
当固定组织的碎片在血清中培养时，细胞增殖和细胞死亡都被降低到极低的水平。
血浆凝块的存在对于在血清中培养的组织的持续存活和进一步发育并不是必需的。
在体外，凝结血浆中的纤维蛋白原、凝血酶原和纤维蛋白对于结缔组织纤维的发育并不是必需的。
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