通过电穿孔法对淡水柄杆菌和海洋柄杆菌进行转化。
Transformation of freshwater and marine caulobacters by electroporation.
作者信息
Gilchrist A, Smit J
机构信息
Department of Microbiology, University of British Columbia, Vancouver, Canada.
出版信息
J Bacteriol. 1991 Jan;173(2):921-5. doi: 10.1128/jb.173.2.921-925.1991.
Abstract
We performed plasmid electrotransformation of Caulobacter crescentus strains and obtained up to 3 x 10(8) transformants per micrograms of pKT230. The presence and integrity of the paracrystalline protein surface (S) layer influenced electroporation; caulobacters lacking the S layer were electrotransformed 10 times more efficiently than caulobacters possessing the S layers. A procedure yielding 1,500 transformants per micrograms of pKT230 was developed for a marine caulobacter. Electroporation was used in combination with several genetic techniques, including introduction of ligation mixtures, suicide transposon mutagenesis, gene replacement, and plasmid electrotransfer from Escherichia coli to caulobacters.
摘要
我们对新月柄杆菌菌株进行了质粒电转化，每微克pKT230最多可获得3×10⁸个转化子。准晶体蛋白表面（S）层的存在和完整性影响电穿孔；缺乏S层的柄杆菌的电转化效率比具有S层的柄杆菌高10倍。针对一种海洋柄杆菌开发了一种每微克pKT230可产生1500个转化子的方法。电穿孔与多种遗传技术结合使用，包括引入连接混合物、自杀转座子诱变、基因替换以及从大肠杆菌到柄杆菌的质粒电转移。
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本文引用的文献
1
Generalized Transduction in CAULOBACTER CRESCENTUS.
Genetics. 1977 Nov;87(3):391-9. doi: 10.1093/genetics/87.3.391.
2
Isolation and Characterization of Marine Caulobacters and Assessment of Their Potential for Genetic Experimentation.
Appl Environ Microbiol. 1988 Mar;54(3):809-817. doi: 10.1128/aem.54.3.809-817.1988.
3
Transposon Tn5 encodes streptomycin resistance in nonenteric bacteria.
J Bacteriol. 1984 Jul;159(1):388-9. doi: 10.1128/jb.159.1.388-389.1984.
4
Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas.
Gene. 1981 Dec;16(1-3):237-47. doi: 10.1016/0378-1119(81)90080-9.
5
Transposon mutagenesis in Caulobacter crescentus.
J Bacteriol. 1982 Feb;149(2):620-5. doi: 10.1128/jb.149.2.620-625.1982.
6
Cloning of the major protein of the Caulobacter crescentus periodic surface layer: detection and characterization of the cloned peptide by protein expression assays.
J Bacteriol. 1984 Dec;160(3):1137-45. doi: 10.1128/jb.160.3.1137-1145.1984.
7
Periodic surface array in Caulobacter crescentus: fine structure and chemical analysis.
J Bacteriol. 1981 Jun;146(3):1135-50. doi: 10.1128/jb.146.3.1135-1150.1981.
8
Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA.
J Mol Biol. 1966 Mar;16(1):118-33. doi: 10.1016/s0022-2836(66)80267-x.
9
Single-stranded hexameric linkers: a system for in-phase insertion mutagenesis and protein engineering.
Gene. 1985;37(1-3):111-23. doi: 10.1016/0378-1119(85)90263-x.
10
High-voltage electroporation of bacteria: genetic transformation of Campylobacter jejuni with plasmid DNA.
Proc Natl Acad Sci U S A. 1988 Feb;85(3):856-60. doi: 10.1073/pnas.85.3.856.
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