Furue M, Katz S I, Kawakami Y, Kawakami T
Dermatology Branch, National Cancer Institute, Bethesda, MD 20892.
J Immunol. 1990 Jan 15;144(2):736-9.
Activation of T lymphocytes induces transcription of several important genes which encode lymphokines and lymphokine receptors as well as "proliferation complementary" protooncogenes like c-myc or c-fos. Recently, the expression of lck gene, one of the src family gene, has also been shown to be modulated during T cell activation. We, therefore, assessed the question of whether other src family genes are expressed during the activation of T cells and of whether cyclosporine, a potent immunosuppressive drug, affects expression of these genes. We examined the expression of four different src family genes (lck, c-src, fyn, and c-fgr) in addition to the expression of IL-2, c-fos, c-myc, and actin genes in murine T cells which were activated with PMA plus ionomycin or PMA plus anti-CD3 mAb. We found that T cell activation was associated with the up-regulation of these src family genes and that the expression of these genes was specifically blocked in the presence of cyclosporine indicating that these activation-related genes were coordinately regulated.
T淋巴细胞的激活会诱导几个重要基因的转录，这些基因编码淋巴因子、淋巴因子受体以及诸如c-myc或c-fos等“增殖互补”原癌基因。最近，src家族基因之一的lck基因的表达也已被证明在T细胞激活过程中受到调节。因此，我们评估了在T细胞激活过程中是否有其他src家族基因表达，以及强效免疫抑制药物环孢素是否会影响这些基因的表达。我们检测了除IL-2、c-fos、c-myc和肌动蛋白基因表达外的四种不同src家族基因（lck、c-src、fyn和c-fgr）在经佛波酯（PMA）加离子霉素或PMA加抗CD3单克隆抗体激活的小鼠T细胞中的表达。我们发现T细胞激活与这些src家族基因的上调相关，并且在环孢素存在的情况下这些基因的表达被特异性阻断，这表明这些与激活相关的基因受到协同调节。
