Laboratories of The Rockefeller Institute for Medical Research.
J Gen Physiol. 1937 Mar 20;20(4):589-94. doi: 10.1085/jgp.20.4.589.
Spores and vegetative forms of a strain of B. megatherium were irradiated by ultraviolet light of the wave lengths 2536 A, 2803 A, and 3132 A. The killing rate of both bacteria and spores is exponential, in agreement with irradiation results on other bacteria. Twice as much incident energy is needed to kill the spores as the vegetative forms (50 per cent death). The absorbed energy per bacterium for 50 per cent killing has been calculated on the assumption that the absorption of the vegetative cells is the same as that of colon bacilli. These results are compared with previous measurements on other bacteria.
用波长为 2536A、2803A 和 3132A 的紫外线照射巴氏芽孢杆菌的芽孢和营养体。细菌和芽孢的致死率均呈指数级,这与对其他细菌的辐照结果一致。杀死芽孢所需的入射能量是杀死营养体的两倍(半致死剂量)。假设营养细胞的吸收与大肠杆菌相同,根据 50%致死量计算了每个细菌吸收的能量。将这些结果与以前对其他细菌的测量结果进行了比较。