Biochemical Research Laboratory, Massachusetts General Hospital, and the Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts 02114.
J Gen Physiol. 1969 Jul 1;54(1):3-26. doi: 10.1085/jgp.54.1.3.
The general relations between protein conformation and the optical activity of peptide chromophores are outlined and applied to the analysis of the optical rotatory dispersion and circular dichroism of the plasma membranes of human erythrocytes and Ehrlich ascites carcinoma cells. It is concluded that the proteins of these membranes are "globular" and that they have considerable helical content. The spectroscopic consequences of perturbing the membranes with phospholipase C, phospholipase A, lysolecithin, and sodium dodecyl sulfate are examined in the light of the effects of these agents upon certain enzymatic and physical properties of the membranes and upon their proton magnetic resonance spectra. The data suggest that the architecture of membrane proteins is strongly dependent upon apolar lipid-protein and/or lipid-sensitive protein-protein interactions.
概述了蛋白质构象与肽生色团的旋光活性之间的一般关系，并将其应用于人红细胞和艾氏腹水癌细胞的血浆膜的旋光色散和圆二色性的分析。结论是这些膜中的蛋白质是“球形”的，并且具有相当大的螺旋含量。根据这些试剂对膜的某些酶和物理性质以及对其质子磁共振谱的影响，检查了用磷脂酶 C、磷脂酶 A、溶血卵磷脂和十二烷基硫酸钠扰动膜的光谱后果。这些数据表明，膜蛋白的结构强烈依赖于非极性脂质-蛋白和/或脂质敏感的蛋白-蛋白相互作用。
