膜悬浮液的旋光性：基于米氏散射理论的假象计算
Optical activity of membrane suspensions: calculation of artifacts by Mie scattering theory.
作者信息
Gordon D J, Holzwarth G
出版信息
Proc Natl Acad Sci U S A. 1971 Oct;68(10):2365-9. doi: 10.1073/pnas.68.10.2365.
Abstract
The circular dichroism, optical rotatory dispersion, and optical density of a suspension of erythrocyte ghosts are calculated from the measured optical properties of solubilized ghosts by classical general scattering theory (Mie theory). The ghost is represented by a solvent-filled spherical shell 7 nm (70 A) thick and 3.5 mum in radius. The 3- to 5-nm red shifts and unusual band shapes observed in the circular dichroism and optical rotary dispersion of suspensions of the intact ghosts, but not in the solubilized membranes, are reproduced by these calculations. Both differential absorption and differential scattering of left-and right-circularly polarized light contribute significantly to the calculated circular dichroism spectra. The artifacts of small membrane vesicles are shown to be less than those of intact ghosts. It is concluded that the characteristic anomalies in the optical activity of membrane suspensions are artifactual.
摘要
利用经典的广义散射理论（米氏理论），根据溶解的红细胞膜空壳的测量光学性质，计算红细胞膜空壳悬浮液的圆二色性、旋光色散和光密度。膜空壳由一个半径为3.5μm、厚7nm（70埃）的充满溶剂的球壳表示。这些计算再现了完整膜空壳悬浮液的圆二色性和旋光色散中观察到的3至5nm红移和异常的带状形状，但在溶解的膜中未观察到。左旋和右旋圆偏振光的差分吸收和差分散射对计算出的圆二色性光谱都有显著贡献。结果表明，小膜泡的伪像比完整膜空壳的伪像要小。得出的结论是，膜悬浮液光学活性中的特征异常是人为造成的。
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