[一株抑制密执安棒杆菌密执安亚种的放线菌菌株的鉴定与分析]
[Identification and analysis of an actinomycete strain suppressing Clavibacter michiganensis subsp, michiganensis].
作者信息
Zhang Yan, Zhang Weihong, Wang Songhong, Li Yaning, Zhao Zhiquan, Liu Daqun, Yang Wenxiang
机构信息
College of Plant Protection, Agricultural University of Hebei, Biological Control Center for Plant Disease and Plant Pests of Hebei Province, Baoding 071001, China.
出版信息
Wei Sheng Wu Xue Bao. 2009 Jul;49(7):889-95.
OBJECTIVE
To identify and analyze bioactive compounds of an actinomycete strain Z-L-22 suppressing Clavibacter michiganensis subsp. michiganensis, the causal agent of bacterial canker of tomato.
METHODS
Morphological, biological and biochemical characterization, chemotaxonomy analysis and 16S rDNA sequences homology analysis were performed to identify the strain Z-L-22. Bioactive compounds were separated and retrieved by thin layer chromatography. Paper chromatography and confirmation tests were used to identify the antibiotic. PCR was carried out using the primers targeted to synthetase of the antibiotic.
RESULTS
Strain Z-L-22 belonged to Streptomyces sp. and was similar to Streptomyces setonii. Two main bioactive components were isolated by thin layer chromatography, which were all identified as actinomycin. New actinomycin synthetase gene was cloned using the primers designed from actinomycin synthetase conserve domain.
CONCLUSION
Strain Z-L-22 was classified as Streptomyces setonii. Actinomycin produced by Streptomyces setonii was first reported.
目的
鉴定并分析一株对番茄细菌性溃疡病病原菌密歇根棒杆菌密歇根亚种具有抑制作用的放线菌菌株Z-L-22的生物活性化合物。
方法
对菌株Z-L-22进行形态、生物学和生化特性鉴定、化学分类分析以及16S rDNA序列同源性分析。通过薄层色谱法分离并提取生物活性化合物。采用纸色谱法和确证试验鉴定抗生素。使用针对该抗生素合成酶的引物进行PCR。
结果
菌株Z-L-22属于链霉菌属且与西托链霉菌相似。通过薄层色谱法分离出两种主要生物活性成分，均鉴定为放线菌素。利用从放线菌素合成酶保守结构域设计的引物克隆了新的放线菌素合成酶基因。
结论
菌株Z-L-22被归类为西托链霉菌。首次报道了西托链霉菌产生放线菌素。
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