Department of Basic Sciences, Dr YS P University of Horticulture and Forestry, Nauni, Solan, 173 230, HP, India.
Department of Soil Science and Water Management , Dr YS P University of Horticulture and Forestry, Nauni, Solan, 173 230, HP, India.
Microb Pathog. 2019 May;130:196-203. doi: 10.1016/j.micpath.2019.03.006. Epub 2019 Mar 13.
A total of 150 rhizobacteria and endorhizobacteria previously isolated from three different horticultural crops; strawberry, apple and apricot were screened for antagonistic activitiy against Clavibacter michiganensis ssp. michiganensis. Among them strain S1, exhibiting significantly higher antagonistic and plant growth promoting ability was characterized as Bacillus amyloliquefaciens based on morphological, biochemical and partial gene sequence analysis of 16S rRNA. B. amyloliquefaciens strain S1 showed maximum growth inhibition of C. michiganensis (12 mm). Moreover, B. amyloliquefaciens strain S1 exhibit significant phosphorus solubilization (94.16 %SEl) and indole acetic acid (27 μg ml) production under in vitro conditions. Antagonistic activity of Bacillus amyloliquefaciens strain S1 was compared with other four strains KUS1, RS, RG and AG against bacterial canker of tomato under net house conditions. Minimum bacterial canker disease incidence (30.0%) was recorded in B. amyloliquefaciens S1 followed by RG after 30 days of inoculation. The bio-control efficacy was higher in B. amyloliquefaciens S1 treated plants, followed by RG.
从三种不同园艺作物（草莓、苹果和杏）中分离得到的 150 株根际和内生细菌，对其进行了拮抗活性筛选，以对抗密歇根棒杆菌亚种。其中，菌株 S1 表现出显著更高的拮抗和植物生长促进能力，根据 16S rRNA 的形态学、生物化学和部分基因序列分析，鉴定为解淀粉芽孢杆菌。解淀粉芽孢杆菌 S1 对 C. michiganensis 的最大生长抑制作用为 12mm。此外，在体外条件下，解淀粉芽孢杆菌 S1 表现出显著的磷溶解（94.16%SEl）和吲哚乙酸（27μg/ml）的产生。在网室内条件下，将解淀粉芽孢杆菌 S1 与其他 4 株菌株 KUS1、RS、RG 和 AG 进行了比较，以评估其对番茄溃疡病的拮抗活性。在接种 30 天后，解淀粉芽孢杆菌 S1 处理的植株溃疡病发病率最低（30.0%），其次是 RG。解淀粉芽孢杆菌 S1 处理的植株的生物防治效果更高，其次是 RG。
