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氨基酸、ATP、焦磷酸和tRNA对氨基烷基腺苷酸与大肠杆菌MRE 600异亮氨酰-tRNA合成酶结合的影响。

Influences of amino acid, ATP, pyrophosphate and tRNA on binding of aminoalkyl adenylates to isoleucyl-tRNA synthetase from Escherichia coli MRE 600.

作者信息

Flossdorf J, Marutzky R, Kula M R

出版信息

Nucleic Acids Res. 1977 Jul;4(7):2455-66. doi: 10.1093/nar/4.7.2455.

DOI:10.1093/nar/4.7.2455
PMID:198742
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342578/
Abstract

Aminoalcohol-AMP esters, structurally related to the assumed intermediates of the amino acid activation reaction, behave as competitive inhibitors both with respect to the amino acid and ATP, when tested in the ATP-(32P) PPi-exchange or the tRNA-charging reaction. However, closer investigation of the binding of norvalinyl adenylate to isoleucyl-tRNA synthetase from Escherichia coli MRE 600 by an equilibrium method shows that only the amino acid is a true competitor, while ATP cannot displace the ester from binding. Pyrophosphate enhances the stability of the ester-enzyme complex whereas tRNA is without detectable influence.

摘要

氨基醇-AMP酯在结构上与假定的氨基酸活化反应中间体相关，当在ATP-(32P)PPi交换或tRNA充电反应中进行测试时，它对氨基酸和ATP均表现为竞争性抑制剂。然而，通过平衡法对来自大肠杆菌MRE 600的异亮氨酰-tRNA合成酶与正缬氨酰腺苷酸的结合进行更深入研究表明，只有氨基酸是真正的竞争者，而ATP不能从结合中取代酯。焦磷酸增强了酯-酶复合物的稳定性，而tRNA则没有可检测到的影响。

相似文献

1
Influences of amino acid, ATP, pyrophosphate and tRNA on binding of aminoalkyl adenylates to isoleucyl-tRNA synthetase from Escherichia coli MRE 600.氨基酸、ATP、焦磷酸和tRNA对氨基烷基腺苷酸与大肠杆菌MRE 600异亮氨酰-tRNA合成酶结合的影响。
Nucleic Acids Res. 1977 Jul;4(7):2455-66. doi: 10.1093/nar/4.7.2455.
2
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本文引用的文献

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Repeating sequences in aminoacyl-tRNA synthetases.氨酰-tRNA合成酶中的重复序列。
Nature. 1974 May 24;249(455):316-20. doi: 10.1038/249316a0.
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Structural studies on isoleucyl-tRNA synthetase from E. coli.
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Ultracentrifuge studies on binding of aliphatic amino acids to isoleucyl-tRNA synthetase from Escherichia coli MRE 600.关于脂肪族氨基酸与大肠杆菌MRE 600异亮氨酰-tRNA合成酶结合的超速离心研究。
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Isolation and properties of isoleucyl-tRNA synthetase from Escherichia coli MRE 600.大肠杆菌MRE 600异亮氨酰-tRNA合成酶的分离与特性
Eur J Biochem. 1973 Jul 16;36(2):528-33. doi: 10.1111/j.1432-1033.1973.tb02939.x.
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The mechanism of action of methionyl-tRNA synthetase from Escherichia coli. Mechanism of the amino-acid activation reaction catalyzed by the native and the trypsin-modified enzymes.来自大肠杆菌的甲硫氨酰 - tRNA合成酶的作用机制。天然酶和胰蛋白酶修饰酶催化的氨基酸活化反应机制。
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On the active site topography of isoleucyl transfer ribonucleic acid synthetase of Escherichia coli B.关于大肠杆菌B异亮氨酰转移核糖核酸合成酶的活性位点拓扑结构
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2-p-Toluidinylnaphthalene-6-sulfonate, a fluorescent reporter group for L-isoleucyl-tRNA synthetase.2-对甲苯胺基萘-6-磺酸盐，一种用于L-异亮氨酰-tRNA合成酶的荧光报告基团。
Biochem Biophys Res Commun. 1971 Oct 15;45(2):409-15. doi: 10.1016/0006-291x(71)90834-5.
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Isoleucyl-tRNA synthetase. A fluorescence study of the binding properties of the synthetase from Escherichia coli.异亮氨酰 - 转运RNA合成酶。大肠杆菌合成酶结合特性的荧光研究。
Eur J Biochem. 1971 May 11;20(1):1-13. doi: 10.1111/j.1432-1033.1971.tb01355.x.
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Isoleucyl transfer ribonucleic acid synthetase. The role of magnesium in amino acid activation.异亮氨酰转移核糖核酸合成酶。镁在氨基酸活化中的作用。
Biochemistry. 1970 Aug 4;9(16):3143-8. doi: 10.1021/bi00818a005.
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On the rate law and mechanism of the adenosine triphosphate--pyrophosphate isotope exchange reaction of amino acyl transfer ribonucleic acid synthetases.关于氨酰基转移核糖核酸合成酶的三磷酸腺苷-焦磷酸同位素交换反应的速率定律和机制。
Biochemistry. 1970 Feb 3;9(3):480-9. doi: 10.1021/bi00805a005.