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1
On the binding of aminoalkyl adenylates to isoleucyl-tRNA synthetase from Escherichia coli MRE 600.氨基烷基腺苷酸与大肠杆菌MRE 600异亮氨酰-tRNA合成酶的结合
Nucleic Acids Res. 1977 Mar;4(3):673-83. doi: 10.1093/nar/4.3.673.
2
Influences of amino acid, ATP, pyrophosphate and tRNA on binding of aminoalkyl adenylates to isoleucyl-tRNA synthetase from Escherichia coli MRE 600.氨基酸、ATP、焦磷酸和tRNA对氨基烷基腺苷酸与大肠杆菌MRE 600异亮氨酰-tRNA合成酶结合的影响。
Nucleic Acids Res. 1977 Jul;4(7):2455-66. doi: 10.1093/nar/4.7.2455.
3
The mechanism of aminoacylation of transfer ribonucleic acid. Reactivity of enzyme-bound isoleucyl adenylate.转移核糖核酸的氨酰化机制。酶结合异亮氨酰腺苷酸的反应活性。
J Biol Chem. 1975 May 25;250(10):3854-60.
4
Influence of side-chain structure of aliphatic amino acids on binding to isoleucyl-tRNA synthetase from Escherichia coli MRE 600.脂肪族氨基酸侧链结构对与来自大肠杆菌MRE 600的异亮氨酰-tRNA合成酶结合的影响。
Eur J Biochem. 1976 Jun 15;66(1):147-55. doi: 10.1111/j.1432-1033.1976.tb10435.x.
5
Direct determination of the enthalpy of binding of tRNAIle to isoleucyl-tRNA synthetase of E. coli MRE 600.直接测定大肠杆菌MRE 600的异亮氨酰-tRNA合成酶与异亮氨酸tRNA（tRNAIle）结合的焓。
Hoppe Seylers Z Physiol Chem. 1980;361(2):201-5.
6
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Eur J Biochem. 1988 Apr 5;173(1):27-34. doi: 10.1111/j.1432-1033.1988.tb13962.x.
7
Interacting binding sites of isoleucyl-tRNA synthetase from Escherichia coli studied by equilibrium partition.通过平衡分配研究大肠杆菌异亮氨酰 - tRNA合成酶的相互作用结合位点。
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8
Isoleucyl-tRNA synthetase from Escherichia coli MRE 600. Different pathways of the aminoacylation reaction depending on presence of pyrophosphatase, order of substrate addition in the pyrophosphate exchange, and substrate specificity with regard to ATP analogs.来自大肠杆菌MRE 600的异亮氨酰-tRNA合成酶。取决于焦磷酸酶的存在、焦磷酸交换中底物添加顺序以及ATP类似物的底物特异性的氨酰化反应的不同途径。
Eur J Biochem. 1982 Nov 15;128(2-3):315-29.
9
Isoleucyl-tRNA synthetase from Escherichia coli MRE 600: discrimination between isoleucine and valine with modulated accuracy.来自大肠杆菌MRE 600的异亮氨酰-tRNA合成酶：对异亮氨酸和缬氨酸的区分及精确性调控
Biol Chem Hoppe Seyler. 1987 Mar;368(3):229-37. doi: 10.1515/bchm3.1987.368.1.229.
10
Mechanism of aminoacylation of tRNA. Proof of the aminoacyl adenylate pathway for the isoleucyl- and tyrosyl-tRNA synthetases from Escherichia coli K12.tRNA的氨酰化机制。来自大肠杆菌K12的异亮氨酰-tRNA合成酶和酪氨酰-tRNA合成酶的氨酰腺苷酸途径的证据。
Biochemistry. 1976 Feb 24;15(4):818-23. doi: 10.1021/bi00649a014.

引用本文的文献

1
Phosphonate analogues of aminoacyl adenylates.氨酰腺苷酸的膦酸酯类似物。
Biochem J. 1978 Nov 1;175(2):461-5. doi: 10.1042/bj1750461.
2
Influences of amino acid, ATP, pyrophosphate and tRNA on binding of aminoalkyl adenylates to isoleucyl-tRNA synthetase from Escherichia coli MRE 600.氨基酸、ATP、焦磷酸和tRNA对氨基烷基腺苷酸与大肠杆菌MRE 600异亮氨酰-tRNA合成酶结合的影响。
Nucleic Acids Res. 1977 Jul;4(7):2455-66. doi: 10.1093/nar/4.7.2455.

本文引用的文献

1
SYNTHESIS OF NUCLEOTIDE ANHYDRIDES BY ANION EXCHANGE.通过阴离子交换合成核苷酸酐。
Biochim Biophys Acta. 1964 Sep 11;91:1-13. doi: 10.1016/0926-6550(64)90164-1.
2
The determination of enzyme inhibitor constants.酶抑制剂常数的测定
Biochem J. 1953 Aug;55(1):170-1. doi: 10.1042/bj0550170.
3
On the binding of chitin oligosaccharides to lysozyme.关于几丁质寡糖与溶菌酶的结合
Proc Natl Acad Sci U S A. 1966 Jul;56(1):26-30. doi: 10.1073/pnas.56.1.26.
4
Some questions about the structure and activity of aminoacyl-tRNA synthetases.关于氨酰-tRNA合成酶的结构与活性的一些问题。
Adv Enzymol Relat Areas Mol Biol. 1971;35:443-501. doi: 10.1002/9780470122808.ch8.
5
[Study of methionyl tRNA synthetase of Escherichia coli. 2. Selective and reversible inactivation of the capacity to activate tRNA].[大肠杆菌甲硫氨酰tRNA合成酶的研究。2. 激活tRNA能力的选择性和可逆失活]
Eur J Biochem. 1968 Apr 3;4(2):222-4. doi: 10.1111/j.1432-1033.1968.tb00197.x.
6
The mechanism of reaction of methionyl-tRNA synthetase from Escherichia coli. Interaction of the enzyme with ligands of the amino-acid-activation reaction.大肠杆菌甲硫氨酰 - tRNA合成酶的反应机制。该酶与氨基酸激活反应配体的相互作用。
Eur J Biochem. 1972 Jan 21;24(3):461-9. doi: 10.1111/j.1432-1033.1972.tb19707.x.
7
Tyrosyl transfer ribonucleic acid synthetase from Escherichia coli B. Analysis of tyrosine and adenosine 5'-triphosphate binding sites.来自大肠杆菌B的酪氨酰转移核糖核酸合成酶。酪氨酸和腺苷5'-三磷酸结合位点的分析。
J Med Chem. 1973 Mar;16(3):273-80. doi: 10.1021/jm00261a025.
8
The mechanism of action of methionyl-tRNA synthetase from Escherichia coli. 1. Fluorescence studies on tRNAMet binding as a function of ligands, ions and pH.来自大肠杆菌的甲硫氨酰 - tRNA合成酶的作用机制。1. 作为配体、离子和pH函数的tRNAMet结合的荧光研究。
Eur J Biochem. 1973 Jul 2;36(1):213-26. doi: 10.1111/j.1432-1033.1973.tb02903.x.
9
Ultracentrifuge studies on binding of aliphatic amino acids to isoleucyl-tRNA synthetase from Escherichia coli MRE 600.关于脂肪族氨基酸与大肠杆菌MRE 600异亮氨酰-tRNA合成酶结合的超速离心研究。
Eur J Biochem. 1973 Jul 16;36(2):534-40. doi: 10.1111/j.1432-1033.1973.tb02940.x.
10
Isolation and properties of isoleucyl-tRNA synthetase from Escherichia coli MRE 600.大肠杆菌MRE 600异亮氨酰-tRNA合成酶的分离与特性
Eur J Biochem. 1973 Jul 16;36(2):528-33. doi: 10.1111/j.1432-1033.1973.tb02939.x.

氨基烷基腺苷酸与大肠杆菌MRE 600异亮氨酰-tRNA合成酶的结合

On the binding of aminoalkyl adenylates to isoleucyl-tRNA synthetase from Escherichia coli MRE 600.

作者信息

Flossdorf J, Marutzky R, Messer K, Kula M R

出版信息

Nucleic Acids Res. 1977 Mar;4(3):673-83. doi: 10.1093/nar/4.3.673.

DOI:10.1093/nar/4.3.673
PMID:325520
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342471/
Abstract

The binding of nine aminoalkyl adenylates to isoleucyl-tRNA synthetase from Escherichia coli MRE 600 was measured and compared with the binding of the cognate amino acids. It was found that they bind rather tightly to the enzyme, the Kd's ranging from 3.1.10(-4) M with glycinol-AMP ester to 3.7.10(-9) M with L-isoleucinol-AMP ester. The binding is not affected by magnesium. It is shown that the free energies of binding of the esters can be calculated adding a constant contribution of the AMP-moiety of about - 4.1 (- 17) kcal/mole (kJ/mole) to the free energies of binding of the cognate amino acids, which we have reported earlier (19, 25, 26).

摘要

测定了9种氨基烷基腺苷酸与大肠杆菌MRE 600异亮氨酰 - tRNA合成酶的结合情况，并与同源氨基酸的结合进行了比较。发现它们与该酶结合相当紧密，解离常数（Kd）范围从甘氨醇 - AMP酯的3.1×10⁻⁴ M到L - 异亮氨醇 - AMP酯的3.7×10⁻⁹ M。这种结合不受镁的影响。结果表明，通过向我们之前报道的（19, 25, 26）同源氨基酸结合自由能中添加约 - 4.1（ - 17）千卡/摩尔（千焦/摩尔）的AMP部分的恒定贡献，可以计算酯的结合自由能。