Bank H L, Schmehl M K, Warner R, Pratt M F, Albernaz M S, Metcalf J S, Darcy M
Beacon Light Center, Sedona, Arizona 86336.
J Surg Res. 1991 Jan;50(1):57-64. doi: 10.1016/0022-4804(91)90010-j.
Local vascular reconstructions frequently require the use of vein grafts to bridge arterial or venous defects. Most previous studies on the use of cryopreserved veins have used relatively large caliber vessels. There have been few studies on the effectiveness of cryopreserved micro- or small-venous allografts. Here, we tested two types of cryopreserved venous allografts: (1) 1.5- to 1.9-mm diameter microvenous grafts (MVG); and (2) 4- to 5-mm diameter small venous grafts (SVG). Cryopreserved MVG allografts were placed into saphenous arteries of six experimental dogs and SVG cryopreserved allografts were placed into femoral arteries of six experimental dogs for 3 to 6 weeks. Two fresh MVG autografts were also transplanted into experimental dogs as controls and autografts were transferred to the contralateral side in SVG dogs as controls. None of the six cryopreserved MVG grafts retained patency but three/six cryopreserved SVG allografts were patent at harvest. Histological examination of grfts revealed control autografts were undergoing arterialization with an intact intima. Experimental cryopreserved allografts showed extensive medial fibrosis, significant lymphocytic infiltrates, and sporadic areas of intact intima for both patent and nonpatent grafts.
局部血管重建常常需要使用静脉移植物来桥接动脉或静脉缺损。此前大多数关于使用冷冻保存静脉的研究都使用了相对较大口径的血管。关于冷冻保存的微小或小静脉同种异体移植物有效性的研究很少。在此，我们测试了两种冷冻保存的静脉同种异体移植物：（1）直径为1.5至1.9毫米的微小静脉移植物（MVG）；以及（2）直径为4至5毫米的小静脉移植物（SVG）。将冷冻保存的MVG同种异体移植物植入6只实验犬的隐动脉中，将冷冻保存的SVG同种异体移植物植入6只实验犬的股动脉中，持续3至6周。还将两根新鲜的MVG自体移植物作为对照移植到实验犬体内，并将SVG犬的自体移植物转移至对侧作为对照。6根冷冻保存的MVG移植物均未保持通畅，但6根冷冻保存的SVG同种异体移植物中有3根在取材时仍保持通畅。移植物的组织学检查显示，对照自体移植物正在进行动脉化，内膜完整。实验性冷冻保存的同种异体移植物显示出广泛的中层纤维化、明显的淋巴细胞浸润，并且无论是通畅还是不通畅的移植物，都有散在的内膜完整区域。
