Narayanan K, Ahn C, Monstrey S, Tran S, Liang M D
Division of Plastic Surgery, University of Pittsburgh Medical Center, PA.
J Reconstr Microsurg. 1993 Jul;9(4):265-70. doi: 10.1055/s-2007-1006665.
Excellent patency rates are currently established in the use of autogenous veins as interposition vein grafts in microsurgical practice. Allografts may be a viable alternative source of vein grafts. Recent advances in cryobiology have enabled the controlled freezing of tissues with preservation of vital cellular elements. Although several reports have shown the successful use of cryopreserved large-vessel allografts, few have addressed cryopreservation of microvessels (1 to 2 mm in diameter). In this study, the authors have successfully cryopreserved femoral veins in a rat model and transplanted them as interposition vein grafts into arterial defects across major histocompatibility barriers (ACI to Lewis). Short-term patencies (21 days) were determined, and histologic and scanning electron microscopic analysis were performed. Patency of 100 percent was achieved in both fresh control veins and in cryopreserved veins at 21 days.
目前，在显微外科实践中使用自体静脉作为静脉移植体时，已确立了优异的通畅率。同种异体移植可能是静脉移植的一种可行替代来源。低温生物学的最新进展已能够在保存重要细胞成分的情况下对组织进行可控冷冻。尽管有几份报告显示冷冻保存的大血管同种异体移植已成功应用，但很少有涉及微血管（直径1至2毫米）冷冻保存的研究。在本研究中，作者在大鼠模型中成功冷冻保存了股静脉，并将其作为静脉移植体跨主要组织相容性屏障（ACI到Lewis）移植到动脉缺损处。测定了短期通畅率（21天），并进行了组织学和扫描电子显微镜分析。新鲜对照静脉和冷冻保存静脉在21天时的通畅率均达到100%。
